化学发光免疫分析法在梅毒筛查呈反应性无偿献血者归队管理的应用

目的探讨化学发光免疫分析法(CLIA)在梅毒酶联免疫吸附试验(ELISA)检测中呈反应性的无偿献血者归队管理的应用可行性。方法对血站ELISA检测常规筛查梅毒螺旋体抗体呈反应性的血液标本,同时进行CLIA检测。检测结果不一致的标本,进一步采用梅毒螺旋体颗粒凝集试验(TPPA)进行确证实验检测。结果ELISA双试剂阳性标本与CL1A检测结果的符合率明显高于ELISA单试剂阳性标本(P<0.05)。CLIA的假阳性率较低。ELISA检测结果阳性S/CO值越低,则假阳性率越高。结论CLIA可以初步用于梅毒反应性无偿献血者的归队管理。

Objective To investigate the feasibility of chemiluminescence immunoassay(CLIA)in the reactivity management of voluntary blood donors in syphilis enzyme-linked immunosorbent assay(ELISA).Methods Blood samples with treponema paUidum antibody reactivity were routinely screened by ELISA,and CLIA was detected at the same time.The specimens with inconsistent results were further confirmed by treponema pallidum particle agglutination test(TPPA).Results The coincidence rate of double ELISA positive samples was significantly higher than that of single ELISA positive samples(P<0.05).CLIA had a low false positive rate.The lower the POSITIVE S/CO value was,the higher the false positive rate was.Conclusion CLIA can be initially used for the management of syphilis reactive voluntery blood donors.