MicroRNA-200a-3p通过靶点蛋白ZEB2和e钙粘蛋白对膀胱癌细胞的生物学影响

Biological effect of microrNA-200a-3p on bladder cancer cells through the target proteins ZEB2 and E-cadherin

目的分析miR-200a-3p在膀胱癌中的生物学作用。方法选取于2018年3月至2020年3月在本院行膀胱部分切除术或根治性膀胱切除术的9例膀胱癌患者,采用qPCR检测膀胱癌组织中miR-200a-3p水平、组织学分析肿瘤形态、WB法测定蛋白表达情况。结果miR-200a-3p在膀胱癌组织中显著下调。组织学分析结果显示膀胱癌黏膜坏死与肉芽组织增生、多核巨噬细胞聚集和浸润性尿路上皮癌有关。蛋白表达结果显示E-钙粘蛋白显著上调、ZEB2蛋白显著下调。推测miR-200a-3p可与E-cadherin和ZEB2相关调控膀胱癌的侵袭和迁移。结论miR-200a-3p可以作为靶向ZEB2的生物标志物,负调控ZEB2的表达,这些发现将有助于寻找新的膀胱癌治疗靶点。

Objective To investigate the biological role of miR-200a-3p in bladder cancer.Methods 9 patients with bladder tumor were collected between March 2018 and March 2020,treated with the partial or radical cystectomy.The qPCR method was used to detect the level of miR-200a-3p in bladder cancer tissue.The histological analysis of tumor morphology,and WB method was used to determine the protein expression.Results The results showed that miR-200a-3p expression level was obviously decreased in the bladder cancer tissue.Mucosal necrosis of bladder cancer was associated with granulation tissue hyperplasia,multinucleated macrophages and infiltrating urothelial carcinoma.The E-cadherin protein expression level was obviously increased and ZEB2 protein level was obviously decreased in the bladder cancer tissue.The results showed that miR-200a-3p can regulate the invasion and migration of bladder cancer with E-cadherin and ZEB2.Conclusion The E-cadherin and ZEB2 protein could be as bladder cancer biomarkers,and also the miR-200a-3p negative regulating the expression level of ZEB2 protein.These findings will help in the search for new therapeutic targets for bladder cancer.