中老年糖尿病衰弱患者与肠道菌群结构及功能相关性研究

Research of relationship between frailty and gut microbiota on middle-aged and the aged patients with diabetes

肠道菌群在糖尿病合并衰弱的发生发展中起到了重要的作用,因此研究中国中老年糖尿病衰弱人群肠道菌群的结构及功能特征性,具有重要意义。本研究收集30例社区中老年糖尿病患者粪便进行宏基因测序,结合临床数据,分析糖尿病衰弱及衰弱前期与糖尿病非衰弱两组人群菌群结构及功能差异性。结果表明:糖尿病衰弱及衰弱前期人群菌群α多样性有所降低,柯林斯氏菌属(Collinsella)、丁酸弧菌属(Butyricimonas)在衰弱人群中相对丰度较高。糖尿病衰弱及衰弱前期人群富集上调的功能基因分布在组氨酸代谢(Histidine metabolism)、EB病毒感染(Epstein-Barr virus infection)、硫代谢(Sulfur metabolism)和Ⅱ型聚酮生物合成(Biosynthesis of type Ⅱ polyketide products)等,而丁酸代谢(Butanoate metabolism)以及苯丙氨酸代谢(Phenylalanine metabolism)等在该人群中下调。本研究为糖尿病衰弱人群的肠道菌群机制探索奠定了理论基础,同时也为糖尿病衰弱及衰弱前期人群的预防与干预提供了一定的科学依据。

Gut microbiota plays an important role in development of diabetes with frailty. Therefore, it is of great significance to study the structural and functional characteristics of gut microbiota in Chinese with frailty. Totally 30 middle-aged and the aged participants in communities with diabetes were enrolled in this study, and their feces were collected. At the same time, we developed a metagenome analysis to explore the different of the structural and functional characteristics between diabetes with frailty and diabetes without frailty. The results showed the alpha diversity of intestinal microbiota in diabetes with frailty was lower. Collinsella and Butyricimonas were more abundant in diabetes with frailty. The functional characteristics showed that histidine metabolism, Epstein-Barr virus infection, sulfur metabolism, and biosynthesis of type Ⅱ polyketide products were upregulated in diabetes with frailty. Otherwise,butanoate metabolism and phenylalanine metabolism were down-regulated in diabetes with frailty. This research provides theoretical basic for exploring the mechanism of the gut microbiota on the occurrence and development of diabetes with frailty, and provides a basic for prevention and intervention of it.