过表达c-Jun对脐血CD19.CAR-T细胞功能影响初探

The Effect of c-Jun Overexpression on the Function of Cord Blood Derived CD19.CAR-T cells

抗原嵌合受体T细胞(chimeric antigen receptor-T cells,CAR-T)疗法已显示出对血液系统恶性肿瘤的显著疗效,但肿瘤复发问题仍未解决,其中CAR-T细胞在体内扩增能力与活性难以维持是原因之一。前期该课题组已成功制备并获得靶向CD19的脐血CAR-T细胞。为了探讨过表达c-Jun能否有效提高脐血CAR-T细胞(umbilical cord blood (UCB)-derived T lymphocytes,CAR-CBT)扩增能力与持久性,该研究将外源性c-Jun基因片段与CD19.CAR基因同时导入CBT细胞完成基因修饰,制备c-Jun转录因子过表达的CAR-CBT细胞,研究c-Jun过表达对脐血CAR-CBT细胞活性的影响。

Antigen chimeric receptor-T cell therapy has shown significant curative effect on hematological malignancies,but the problem of tumor recurrence has not been solved.One of the reasons is that the expansion and activity of CAR-T cells in vivo are difficult to maintain.In the early stage,research group has successfully prepared CD19 targeted CAR-T cells derived from cord blood (CD19.CAR-CBT cells).To explore whether overexpression of c-Jun can effectively improve the expansion and durability of CAR-CBT cells,the study will exogenous c-Jun gene fragments and CD19.CAR gene into CBT cells to complete genetic modification.Prepare CD19.CAR-CBT cells overexpressing c-Jun transcription factor to study the effect of c-Jun overexpression on the activity of cord blood CAR-CBT cells.