基于重测序的4个秆形变异毛竹变型的全基因组序列分析

Genomic Sequence Analysis of Four Culm Shape Variants of Moso Bamboo Based on Re-sequencing

为揭示秆形变异的毛竹变型全基因组突变类型,以强竹(Phyllostachys pubescens f.obliquinoda)、龟甲竹(Phyllostachys edulis f.heterocycla)、青龙竹(Phyllostachys pubescens f.curviculmi)和圣音竹(Phyllostachys edulis f.tubaeformis)等4个毛竹变型为材料进行全基因组重测序,检测并注释单核苷多态性(SNP)、小片段插入缺失(InDel)和结构变异(SV),运用GO、COG和KEGG等功能数据库比对、注释变异基因,分别有7477、7584、7221和7583个变异基因得到功能注释。GO注释分类包括细胞组件、分子功能和生物过程三个基因功能分类体系的56个功能组。COG注释获得的参与复制、重组和修复的基因数为369个,信号转导机制的基因数为327个,转录的相关基因253个。通过KEGG数据库系统地分析变异基因参与的黄酮类、类胡萝卜素等物质代谢合成途径。深入研究这些差异基因的调控途径,可以为进一步探索毛竹种内丰富的多态性和遗传变异提供一定的理论基础,为开发重要性状分子标记提供重要参考。

To reve al the whole genome differences of Moso bamboo variations on culm shape,Phyllostachys edulis f.obliquinoda,Phyllostachys edulis f.heterocycla,Phyllostachys pubescens f.curviculmi,Phyllostachys edulis f.tubaeformis were re-sequenced by whole genome re-sequencing,to detect and annotate the single nucleotide polymorphism(SNP),insertion-deletion(InDel)and structure variation(SV).The mutant genes were compared with the functional databases of GO,COG,KEGG,of which 7477,7584,7221 and 7583 mutant genes were annotated respectively.GO annotation classification included 56 functional groups of three functional classification systems:cellular component,molecular function and biological processes.COG classification showed that 369 genes involved in replication,recombination and repair,327 genes involved in signal transduction mechanism,and 253 genes involved in transcription.The metabolic pathways of flavonoids,carotenoids and other substances involved in the mutant genes were analyzed by KEGG database.In-depth study of the regulatory pathways can provide a theoretical basis for further exploration of the rich polymorphism and genetic variation of Moso bamboo,and the development of molecular markers for some significant characters.