杨树枝干病害病原物分离与鉴定

Isolation and Identification of Pathogens of Poplar Stem Diseases

自2017年开始,青海省互助县双树苗圃青杨发生大面积枝干坏死,髓心组织枯黄,严重者树皮至髓部有裂纹,夏季有棕褐色粘液流出,髓部黄棕色,无明显病症。本研究对青杨枝干病害进行了症状观察、标本采集,对病原物进行分离培养、形态观察和分子生物学鉴定。对病原物分离培养和纯化后,获得一株细菌纯菌株H_(1),并进行了致病性检测。结果表明,病原细菌H_(1)导致青杨植株感病,与田间发病症状一致。通过形态学观察和分子序列分析鉴定了病原菌H_(1),为Pantoea agglomerans,可引起杨树细菌性溃疡病。本研究明确了互助县青杨枝干病害病原菌,可为该病害防治提供依据。

Since 2017,Populus cathayana in Shuangshu Nursery,Huzhu County,Qinghai Province has suffered from extensive necrosis of branches,yellow pith tissue,cracks from bark to pith in severe cases.In summer,brown mucus has been released,and the pulp is yellow and brown,without obvious disease.In this study,symptom observation,specimen collection,pathogen isolation and culture,morphological observation and molecular biological identification were carried out on poplar branch diseases.After the pathogen was isolated,cultured and purified,a pure bacterial strain H_(1) was obtained and its pathogenicity was tested.The results showed that the pathogenic bacterium H_(1) caused the disease of Populus cathayana plants,which was consistent with the disease symptoms in the field.Through morphological observation and molecular sequence analysis,the pathogen H_(1) was identified as Pantoea.agglomerans,which can cause poplar bacterial canker.This study identified the pathogen of Populus cathayana branch disease in Huzhu country,which can provide basis for the prevention and control of the disease.