响应面优化油茶籽油游离型特征酚和总酚工艺

Optimization of Free Characteristic Phenolic and Total Phenol From Camellia Oleifera Seed Oils by Response Surface Methodology

使用甲醇提取溶剂,采用超声波辅助提取冷榨油茶籽油中以反-肉桂酸和4-羟基苯乙酸为主的游离型多酚化合物。基于单因素实验和响应面分析,优化了游离型特征酚和总酚提取工艺,建立了数学回归模型,分析了各因素间的交互作用。结果表明,影响油茶籽油游离型多酚含量的显著因素为料液比、超声功率和提取时间,最佳工艺为料液比1∶19.73(g/mL)、超声功率265.77 W、提取时间31.86 min,此时总酚、反-肉桂酸和4-羟基苯乙酸含量分别为99.10 mg/kg、520.70μg/kg和348.14μg/kg。验证结果与理论预测拟合度高,可为后期油茶籽油不同形态多酚提取与鉴定、油茶籽油适度加工过程多酚保留与调控提供理论依据。

With methanol as extraction solvent,using ultrasonic-assisted extraction system,the extraction optimization of free polyphenols of trans-cinnamic acid and 4-hydroxyphenylacetic acid from cold pressed Camellia seed oils were studied.On the basis of single-factor experiments,the response surface methodology was adopted to evaluate the free characteristic phenolic and total phenol.Plus,the pairwise interactions of the various extraction conditions were investigated.The results indicated that the solid-liquid ratio,ultrasonic power and extraction time were the most significant factors affecting the free polyphenols in C.Oleifera seed oils.The optimum conditions were solid-liquid ratio being 1∶19.73,ultrasonic power 265.77 W,and extraction time 31.86 min.The predicted content of the free total phenol,trans-cinnamic acid and 4-hydroxyphenylacetic acid under these conditions were 99.10 mg/kg,520.70μg/kg and 348.14μg/kg,respectively.On the basis of the high fitting degree of the verification results and theoretical prediction,these evidences could provide a theoretical basis for the extraction and identification of polyphenol forms in the later stages and the retention and regulation of polyphenols in moderately processed C.Oleifera seed oils.