基于16S rRNA基因测序分析不同喂养方式对食物过敏婴幼儿肠道微生态的影响

Effects of different feeding practices on the intestinal microecology of infants with food allergies based on 16S rRNA gene sequencing analysis

目的通过16S rRNA测序,分析不同喂养方式对食物过敏婴幼儿肠道微生态的影响。方法应用Illumina高通量测序技术检测食物过敏婴幼儿(n=44)及健康婴幼儿(n=12)的粪便中微生物16S rRNA-V3区,分析不同喂养方式的食物过敏婴幼儿肠道菌群的差异。结果(1)人工喂养和混合喂养的食物过敏婴幼儿粪便中平均临时操作单元(OTU)数量,较母乳喂养的食物过敏婴幼儿和健康婴幼儿明显升高,差异有统计学意义(P<0.05);(2)4组婴幼儿间拟杆菌门、梭杆菌门和放线菌门的相对丰度差异有统计学意义(P<0.05);(3)物种注释显示所有样本最佳的分类水平为科,4组间差异贡献最大的类群为瘤胃菌科、双歧杆菌科和拟杆菌科。所有样本进行聚类分析发现,母乳喂养组与对照组、人工喂养组与混合喂养组的相似性较高。结论食物过敏可能与喂养方式所致的肠道微生态的改变有关。

Objective To analyze the effects of different feeding practices on the intestinal microecology of infants with food allergy by 16S rRNA sequencing.Methods The illumina high-throughput sequencing technology was applied to detect the microbial 16S rRNA-V3 region in the faeces of food-allergic infants(44 cases)and healthy infants(12 cases),and to analyse the differences in the intestinal flora of food-allergic infants with different feeding practices.Results(1)The mean number of OTUs in the faeces of hand-fed and mixed-fed food-allergic infants was significantly higher than that of breast-fed food-allergic infants and healthy infants,with statistically significant differences(P<0.05).(2)The relative abundance of the phylum mycobacterium,clostridium and actinomycetes showed significant differences between the four groups(P<0.05).(3)Species annotation showed that the best taxonomic level for all samples was family,and the groups contributing the most to the difference between the four groups were ruminobacteriaceae,bifidobacteriaceae and bacteroidaceae.Cluster analysis of all samples showed that the breastfeeding group and control group,and the artificial feeding group and mixed feeding group had high similarity.Conclusion Food allergy may be associated with alterations in the intestinal microecology due to feeding patterns.