2016-2019年江苏省南京市人感染副溶血弧菌分子特征分析

Molecular analysis on Vibrio parahaemolyticus strains isolated from patients in Nanjing,Jiangsu,2016-2019

目的了解江苏省南京市副溶血弧菌临床分离株的分子特征,为其防控提供参考。方法收集2016—2019年从腹泻和食物中毒患者分离的副溶血弧菌临床分离株并进行血清型分型,运用聚合酶链式反应(PCR)方法检测菌株的tlh、tdh、trh、toxRS/new、ORF8五种毒力基因,采用多位点序列分型(MLST)对菌株进行分子分型分析。结果2016—2019年共收集93株临床分离株,菌株优势血清型为O3∶K6(65/93,69.9%),均携带tlh基因,73株属于tdh+trh-toxRS/new+的大流行菌群,非大流行菌群菌株中有5株为tdh-trh-非致病株。共检出13个ST型,以ST3为主(68/93,73.1%)。结论南京地区副溶血弧菌以O3∶K6、ST3型大流行菌群菌株为主,非大流行菌群、非致病株并存。

Objective To understand the molecular characteristics of Vibrio parahaemolyticus strains in Nanjing,Jiangsu,and provide evidence for the prevention and control of human infection with V.parahaemolyticus.Methods Clinical isolates from diarrhea and food poisoning patients were collected from 2016 to 2019.Virulence genes of tlh,tdh,trh,toxRS/new and ORF8 were detected by polymerase chain reaction(PCR).Molecular types were analyzed by multilocus sequence typing(MLST).Results A total of 93 V.parahaemolyticus strains were collected.O3∶K6 was the predominant serotype(65 strains,69.9%).Virulence genes screening revealed that all isolates carried tlh gene.A total of 73 strains with tdh+trh-toxRS/new+were classified as pandemic strains,and 5 non-pandemic strains with tdh-trh-were non-pathogenic strains.A total of 13 STs were detected,of which ST3 was predominant(68/93,73.1%).Conclusion Molecular analysis on V.parahaemolyticus revealed that ST3 was predominant,and both the pandemic and non-pandemic strains were distributed in Nanjing.