2016-2019年北京市海淀区沙门菌血清分布、分子分型及耐药性分析

Serotype distribution,molecular type,and antimicrobial resistance of Salmonella isolated in Haidian district of Beijing,2016-2019

目的分析北京市海淀区沙门菌血清分布、脉冲场凝胶电泳(PFGE)分型和耐药情况,并找出其相关性,从而掌握海淀区沙门菌的流行特征和耐药现状。方法对2016—2019年北京市海淀区检出的76株沙门菌进行血清分型、PFGE分型和药敏试验。结果肠炎沙门菌菌株占比最高,为59.2%(45/76);76株沙门菌经PFGE聚类分析得到35种带型,各带型之间相似度为48.7%~100.0%,其中,51株沙门菌聚集形成4个簇(带型相似度≥85%);76株沙门菌药物敏感率最高的为亚胺培南100.0%(76/76),中介率最高的为左氧氟沙星80.3%(61/76),耐药率最高的为萘啶酸68.4%(52/76);总体多重耐药率为52.6%(40/76),呈现出27种多重耐药谱。结论2016—2019年北京市海淀区沙门菌血清型和PFGE带型一致性很高,同一血清型菌株PFGE带型相同或相似度很高。相同血清型沙门菌,PFGE带型相同或成簇的菌株间耐药谱有很强的关联性。

Objective To analyze the serotype distribution,PFGE pattern and the antimicrobial resistance of Salmonella isolated from Haidian district of Beijing,and understand the circulating characteristics and antimicrobial resistance of Salmonella in Haidian.Methods Serotyping,PFGE and antimicrobial resistance testing of 76 strains of Salmonella isolated in Haidian between 2016 and 2019 were conducted.Results The proportion of Salmonella enteritidis was highest(59.2%,45/76).In the 76 Salmonella strains,35 PFGE patterns were detected.The similarity between these patterns was 48.7%–100.0%,and 51 strains of Salmonella formed 4 clusters(type similarity≥85%).The 76 strains of Salmonella were all sensitive to imipenem(100.0%,76/76).The intermediary rate to levofloxacin was highest(80.3%,61/76).The resistance rate to nalidixic was highest(68.4%,52/76).The overall multi-drug resistance rate was 52.6%(40/76),showing 27 multi-drug resistance spectrums.Conclusion In the Salmonella strains isolated in Haidian from 2016 to 2019,the serotype and PFGE pattern showed high consistency,the strains with same serotype shared same or highly similar PFGE pattern and a strong association of antimicrobial resistance spectrums exited among the strains with same serotype and same PFGE pattern or in same cluster.