非生物胁迫处理后拟南芥过氧化氢含量变化的分析

Analysis of hydrogen peroxide content changes in Arabidopsis thaliana after abiotic stress treatment

干旱、低温、盐胁迫和重金属污染等非生物胁迫,对植物的生长发育会产生严重的抑制作用.在这些条件下,植物细胞内各种代谢过程会有一定程度的受损或不工作,将会导致活性氧(Reactive oxygen species,ROS)在植物体内过量积累.过氧化氢是植物体内中最稳定形式的活性氧之一,植物体内积累过量的过氧化氢会严重威胁植物细胞的膜系统.在本实验中,选取了野生型拟南芥(Arabidopsis thaliana)为材料,分别进行低温处理、盐处理和干旱处理,并通过对未处理的和非生物胁迫处理的植物中过氧化氢含量变化进行比较分析.实验结果表明,胁迫处理后,拟南芥植物体内的过氧化氢含量不同程度地增加,为深入研究植物对逆境响应的分子机制奠定研究基础.

Abiotic stresses such as drought,low temperature,salt stress and heavy metal contamination can cause severe inhibition of plant growth and development.Under these conditions various metabolic processes within plant cells will be damaged,which will lead to excessive accumulation of reactive oxygen species(ROS)in plants.Hydrogen peroxide(H_(2)O_(2))is one of the most stable forms of ROS,and excessive H_(2)O_(2) accumulation in the plant cellscan seriously threaten the membrane system of plant cells.In this study,wild-type Arabidopsis thaliana was treated with low temperature,salt and drought,respectively.The change of H_(2)O_(2) content between untreated and abiotic stress-treated plants was analyzed.The results showed that H_(2)O_(2) content in Arabidopsis thaliana plants significantly increased after abiotic stress treatments,which provided research foundation for the molecular mechanisms that plant responses to abiotic stress.