幽门螺杆菌不同菌型感染对慢性非萎缩性胃炎血清PG、G-17及IL-10表达的影响

The Influence of Helicobacter Pylori Infection with Different Bacterial Types on the Expression of Serum PG,G-17 and IL-10 in Chronic Non-atrophic Gastritis

目的探讨血清PG、G-17、IL-10在不同菌型幽门螺杆菌(helicobacter pylori,Hp)感染慢性非萎缩性胃炎中的表达及其临床意义。方法随机选取2017年1月—2018年1月经13碳呼气试验、胃镜及其病理组织学检查明确诊断为Hp感染慢性非萎缩性胃炎患者60例为研究对象。受试者抽取血清检测Hp基因抗体(用免疫印迹法测定其血清中Hp CagA、VacA、UreB、UreA抗体),并根据Hp分型标准,对检测结果进行分型分组,CagA、VacA单项或2项阳性者纳入Ⅰ型Hp感染组,UreB、UreA单项或2项阳性者纳入Ⅱ型Hp感染组。两组受试者血清进一步进入第二程序实验室检测,分别采用微孔双抗体夹心法检测血清胃蛋白酶原I(PGI)与胃蛋白酶原Ⅱ(PGⅡ)比值(PGR),采用酶联免疫吸附法检测胃泌素17(G-17)、白介素10(IL-10)。结果60例Hp感染慢性非萎缩性胃炎患者中纳入Ⅰ型Hp感染组45例(75%),Ⅱ型Hp感染者15例(25%)。Ⅰ型Hp感染组中PGR(13.97±4.38)、IL-10指标(35.21±4.48)pg/mL均低于Ⅱ型Hp感染组,两组比较差异有统计学意义(t=2.984、3.156,P<0.05)。两组G-17比较差异无统计学意义(P>0.05)。结论Hp感染慢性非萎缩性胃炎患者以Ⅰ型Hp感染为主;不同菌型Hp感染对胃泌素分泌的影响大致相当;Ⅰ型Hp感染组PGR比值及IL-10表达水平均低于Ⅱ型Hp感染组,提示Ⅰ型Hp感染组胃黏膜抗炎免疫防御功能低于Ⅱ型Hp感染组。

Objective To investigate the expression and clinical significance of serum PG,G-17 and IL-10 in chronic non-atrophic gastritis infected with different types of Helicobacter pylori(Hp).Methods From January 2017 to January 2018,60 patients with chronic non-atrophic gastritis diagnosed as Hp infection by 13 carbon breath test,gastroscope and histopathological examination were randomly selected as the research objects.Subjects draw serum to detect Hp gene antibodies(determine Hp CagA,VacA,UreB,UreA antibodies in their serum by Western blotting).And according to the Hp typing standard,the test results were classified and grouped.CagA,VacA single or two positives were included in the type I Hp infection group,UreB,UreA single or two positives were included in the type Ⅱ Hp infection group.The sera of the two groups of subjects were further tested in the second procedure laboratory,and the ratio of pepsinogen Ⅰ (PG Ⅰ )to pepsinogen Ⅱ (PG Ⅱ )(PGR)was detected by the microporous double antibody sandwich method,and the enzyme-linked immunosorbent assay method to detect gastrin 17(G-17)and interleukin 10(IL-10).The test results were processed and analyzed by statistical methods.Results Among the 60 patients with chronic non-atrophic gastritis with Hp infection,45 patients(75%)with type Ⅰ Hp infection and 15 patients(25%)with type Ⅱ Hp infection were included.The PGR(13.97±4.38)and IL-10 index(35.21±4.48)pg/mL in the type Ⅰ Hp infection group were lower than those in the type Ⅱ Hp infection group,and the difference between the two groups was statistically significant(t=2.984,3.156,P<0.05).There was no statistically significant difference in G-17 between the two groups(P>0.05).Conclusion Hp infection patients with chronic non-atrophic gastritis are mainly type Ⅰ Hp infection.Different bacterial types of Hp infection have roughly the same effect on gastrin secretion.The PGR ratio and IL-10 expression level of the type I Hp infection group were lower than those of the type Ⅱ Hp infection group,suggesting that the gastric mucosal anti-inflammatory immune defense function of the type I Hp infection group was lower than that of the type Ⅱ Hp infection group.