睾酮对水牛卵泡颗粒细胞雌激素合成能力的影响

Effects of testosterone on estrogen synthesis competence of buffalo granulosa cells

为了系统研究雄激素对水牛卵泡颗粒细胞类固醇激素合成的影响,本研究采用在颗粒细胞培养液中添加睾酮的方法,探讨睾酮对水牛颗粒细胞雌激素合成的影响。结果表明:添加10^(7)mol/L睾酮到水牛颗粒细胞培养液中能显著提高水牛颗粒细胞雌激素合成相关基因(CYP11A1、CYP19A1、3β-HSD和17β-HSD)表达和雌二醇分泌水平(P<0.05);且睾酮添加到水牛颗粒细胞培养液中48或72 h能显著提高水牛颗粒细胞雌激素合成相关基因(CYP11A1、CYP19A1和3β-HSD)表达和雌二醇分泌水平(P<0.05)。综上所述,10^(7)mol/L睾酮处理水牛颗粒细胞48或72 h有利于提高水牛颗粒细胞雌激素的合成能力。本研究为进一步探讨水牛卵泡发育类固醇激素分泌机制提供一定理论依据。

In order to systematically investigate the effects of androgen on steroidogenic competence of buffalo granulosa cells(GCs),GC medium supplemented with testosterone was used to explore the effects of testosterone on the estrogen synthesis of buffalo GCs.These results showed that adding concentration 10^(7)mol/L testosterone to GC medium significantly enhanced both the expression levels of estrogen synthesis-related genes(CYP11A1,CYP19A1,3β-HSD,and 17β-HSD) of and the secretion levels of estradiol in buffalo GCs(P<0.05).The treatment of 48 or 72 h in medium supplemented with testosterone significantly improved both the expression levels of estrogen synthesis-related genes(CYP11A1,CYP19A1,and 3β-HSD) of and the secretion levels of estradiol in buffalo GCs(P<0.05).Testosterone that used at concentration of 10^(7)mol/L and applied to GCs for 48 or 72 h promoted the estrogen synthesis competence of buffalo GCs.This study provides a basis for further exploration of ovarian endocrine mechanism in buffalo.