Synergistic activity of insulin combined with glucose on Toxoplasma gondii proliferation in Vero cells

To the Editor:Toxoplasma gondii is a widespread apicomplexan parasite and can infect virtually all warm-blooded vertebrates.[1]Between 22%and 84%of the population in both developed and developing countries is chronically infected with T.gondii.Due to the ethical issues of using animal models for in vivo culture,cell culture systems have been used.Production of T.gondii tachyzoites in vitro is essential for Toxoplasma inves-tigations.It is of great significance to explore the culture conditions of T.gondii in vitro for the in-depth study of the metabolism mechanisms of eukaryotes.