柿子叶有效部位的抗肿瘤活性研究

ANTITUMOR ACTIVITY OF EFFECTIVE FRACTION OF PERSIMMON LEAVES

以柿子叶为研究对象,采用70%乙醇回流提取柿子叶的有效成分。提取物浓缩后加水搅拌,依次用石油醚、二氯甲烷、乙酸乙酯、正丁醇四种溶剂进行梯度萃取,浓缩各相,加上乙醇粗提物共得到六种干燥浸膏。采用CCK试剂检测法,测定六种浸膏对两组癌细胞人宫颈癌细胞Hela、肺癌细胞A549和正常细胞人肾上皮细胞293的生长抑制作用。结果表明,六种浸膏均具有较强抗肿瘤活性,其中二氯甲烷层和正丁醇层对Hela细胞抑制活性最好,其IC_(50)分别为0.24、0.26 mg/mL,A549细胞则对乙酸乙酯层更为敏感,其IC_(50)值低至0.03 mg/mL。此外,六种浸膏对正常人肾上皮细胞293也显示出明显的抑制作用,尤其是乙酸乙酯层,IC_(50)值达到0.93 mg/mL。研究证实了柿子叶具有明显抗肿瘤活性,且其活性成分复杂,在各相中均有分布,但又对不同细胞株活性各有差异,提示在后续的活性追踪中可以根据活性需求,选择恰当的柿子叶有效部位进行研究。

In this research,the active ingredient of the dried persimmon leaves as study object was extracted by alcohol reflux extraction.The crude extracts were extracted with petroleum ether,dichloromethane,ethyl acetate and n-butanol in turn,and totally six kinds of dry extracts were obtained.CCK assay was used to detect the growth inhibition of six extracts on human cervical cancer cells,lung cancer cells and human renal epithelial cells.The results showed that all the six extracts had strong anti-tumor activity,with IC_(50) of 0.24 and 0.26 mg/mL,respectively.While the A549 cells were more sensitive on the ethyl acetate phase,with IC_(50) of 0.03 mg/mL.What’s more,the six extracts showed inhibition against the normal cells 293,expecially the ethyl acetate phase,which showed the IC_(50) of 0.93 mg/mL against the normal cells 293.This study confirmed that persimmon leaves had obvious anti-tumor activity,and its active components were complex and distributed in each extract phases.The activities of the extract phases against the different cell lines were different.It suggested that in the follow-up activity tracking,the appropriate effective parts could be selected for research according to the research aim.