不同炮制品组方的补阳还五汤抗大鼠脑缺血再灌注损伤保护作用的比较研究

Comparative Study on the Protective Effects of Buyang Huanwu Decoction with Different Processed Products on Cerebral Ischemia-Reperfusion Injury in Rats

目的比较不同炮制品组方的补阳还五汤对脑缺血再灌注损伤的保护作用。方法将175只SPF级SD大鼠随机分为假手术组、模型组、氢氯吡格雷组、方剂1组(各组成成分均为生品)、方剂2组(黄芪蜜炙,当归、赤芍、川芎、地龙均酒炙,其他药物均为生品)、方剂3组(黄芪蜜炙,其他药物均为生品)以及方剂4组(当归、赤芍、川芎、地龙均酒炙,其他药物均为生品),假手术组与模型组给予等量生理盐水灌胃,氢氯吡格雷组按0.68 mg/mL氢氯吡格雷水溶液灌胃,方剂组按13 g·kg^(-1)·d^(-1)量灌胃。采用线栓法建立大鼠大脑中动脉栓塞脑缺血再灌注模型。检测各组的血清乳酸脱氢酶(LDH)活性,丙二醛(MDA)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)水平,以及血清PAF、CD62p、CD63含量。结果与模型组比较,氢氯吡格雷组和方剂1、2、3、4组均可显著抑制大鼠血清LDH活性和MDA、TNF-α、IL-1β、IL-6水平的升高(P<0.01,P<0.05),均可不同程度降低血清PAF、CD62p、CD63的表达水平(P<0.01,P<0.05),其中方剂2组的效果优于方剂3组、方剂4组和氢氯吡格雷组(P<0.05),方剂3组、方剂4组、氢氯吡格雷组间无显著差异(P<0.05)。结论黄芪蜜炙,当归、赤芍、川芎、地龙酒炙组成的补阳还五汤对大鼠脑缺血再灌注损伤具有显著的保护作用,其机制与抑制血小板活化、抑制炎性因子分泌和表达及减轻脑组织炎性反应有关。

Objective To compare the protective effects of Buyang Huanwu decoction with different processed products on cerebral ischemia-reperfusion injury in rats.Methods 175 SPF SD rats were randomly divided into sham operation group,model group,clopidogrel group,prescription 1 group(all the components were raw),prescription 2 group(Astragalus Membranaceus was honey-fried,Chinese Angelica Root,Common Peony Root,Chuanxiong Rhizome and Lumbricus were wine-processed,and other components were raw),prescription 3 group(Astragalus Membranaceus was honey-fried,and other components were raw),and prescription 4 group(Chinese Angelica Root,Common Peony Root,Chuanxiong Rhizome and Lumbricus were wine-processed,and other components were raw).The sham operation group and model group were given the same amount of saline by gavage,and the clopidogrel group was given 0.68 mg/mL clopidogrel aqueous solution by gavage.The prescription groups was gavaged by the dose of 13 g·kg^(-1)·d^(-1).The middle cerebral artery occlusion cerebral ischemia-reperfusion model in rats was established by thread embolism method.Serum lactate dehydrogenase(LDH)activity,levels of malondialdehyde(MDA),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin-6(IL-6),and contents of serum PAF,CD62p and CD63 levels were detected in all groups.Results Compared with the model group,the increased of LDH activity and levels of MDA,TNF-α,IL-1βand IL-6 in serum of rats in clopidogrel group,prescription 1 group,prescription 2 group,prescription 3 group and prescription 4 group were significantly inhibited(P<0.01,P<0.05),and the expression levels of PAF,CD62p and CD63 in serum were decreased to varying degrees(P<0.01,P<0.05).The effects of prescription 2 group were better than those of prescription 3 group,prescription 4 group and clopidogrel group(P<0.05),while no statistically significant difference was found among prescription 3 group,prescription 4 group and clopidogrel group(P<0.05).Conclusions Buyang Huanwu decoction with honey-fried astragalus membranaceus and wine-processed Chinese Angelica Root,Common Peony Root,Chuanxiong Rhizome and Lumbricus has significant protective effect on cerebral ischemia-reperfusion injury in rats.Its mechanism is related to inhibition of platelet activation,inhibition of inflammatory factor secretion and expression,and reduction of inflammatory response in brain tissue.