脐带间充质干细胞及其外泌体对HK-2细胞脂多糖损伤的作用研究

The Effect of Exosomes Derived from HuMSCs and HuMSCs on LPS Injury of HK-2 Cells

目的探讨脐带间充质干细胞及其外泌体对脂多糖(LPS)致肾小管上皮细胞损伤的影响。方法采用10μg LPS处理肾小管上皮细胞,随后分别将脐带间充质干细胞及其外泌体与受损肾小管上皮细胞共培养。24h后MTS法测定各组细胞增殖活力,qRT-PCR和Western blot检测各组TLR4的表达,Elisa检测培养上清液中炎症介质(IL-1β、IL-6、TNF-α)的水平。结果脐带间充质干细胞及其外泌体均可显著促进受损肾小管上皮细胞增殖,抑制LPS导致的HK-2细胞TLR4的表达,并且抑制LPS导致的炎症因子表达升高。结论脐带间充质干细胞及其外泌体促进肾小管上皮细胞增殖,抑制LPS导致的HK-2细胞TLR4的表达,这一作用可能与间充质干细胞及其来源外泌体的旁分泌功能密切相关。

Objective To investigate the effect of human umbilical cord mesenchymal stem cells and their exosomes on the damage of renal tubular epithelial cells induced by LPS.Methods The renal tubular epithelial cells were treated with 10μg LPS,and then the human umbilical cord mesenchymal stem cells and their exosomes were co-cultured with the damaged renal tubular epithelial cells.After 24 hours,the cell proliferation activity of each group was determined by MTS method,the expression of TLR4 in each group was detected by qRT-PCR and WB,and the level of inflammatory mediator(IL-1β,IL-6,TNF-α).Results Human umbilical cord mesenchymal stem cells and their exosomes can significantly promote the proliferation of damaged renal tubular epithelial cells,and inhibit the expression of TLR4 in HK-2 cells caused by LPS,and inhibit the increase in expression of inflammatory factors caused by LPS.Conclusion Human umbilical cord mesenchymal stem cells and their exosomes promote the proliferation of renal tubular epithelial cells and can inhibit the expression of TLR4 in HK-2 cells caused by LPS.The effect may be closely related to the paracrine effect of mesenchymal stem cells.