摘要
Objective:To investigate the relationship between inflammatory polarization and Notch pathway in rats with adjuvant arthritis.Methods:Twelve rats were randomly divided into normal(NC)group(n=6)and model(MC)group(n=6).In the model group,complete Freund's adjuvant(0.1ml/rat)was injected into the right hindfoot to induce inflammation.On the 12th day after inflammation,the changes of plantar swelling degree(E)and arthritis index(AI)were observed,and the expressions of inflammatory polarization markers CD68 and CD206 in peripheral blood were detected by flow cytometry.PCR was used to detect the expression of factors related to Notch signal pathway in peripheral blood.Results:Compared with the normal group,the expression of E,AI,CD68,Notch2,Notch3,Notch4 and Delta1 in the model group increased significantly,while the expression of CD206,Notch1,Jagged1 and Jagged2 decreased(P<0.01or P<0.05).The results showed that CD68,toe swelling degree and arthritis index were negatively correlated with Notch1,Jagged1 and Jagged2,CD68,toe swelling degree and arthritis index were positively correlated with Notch2,Notch4 and Delta1,CD206 was positively correlated with Notch1 and Jagged1,Jagged2 and CD206 was negatively correlated with Notch2,Notch4 and Delta1.Conclusion:Notch signal pathway may promote the occurrence and development of AA by regulating inflammatory polarization of macrophages.
基金
General Program of National Natural Science Foundation of China(No.81973655)
Natural Science Research Project of Colleges and Universities in Anhui Province(No.KJ2020A0397)
National Key Research and Development Program of the Ministry of Science and Technology of Chinese Medicine Modernization Research(No.2018YFC1705204)
National TCM Innovation Key Talents Training Project(No.[2019]128)
Project of Anhui University of Traditional Chinese Medicine(No.2018xrxd17)
Intra-hospital Fund of First Affiliated Hospital,Anhui University of Traditional Chinese Medicine project(No.2020yfyzc25,2020yfyzc55)。
