富血小板血浆联合间充质干细胞治疗早期膝关节炎的临床效果研究

Clinical effect of platelet-rich plasma combined with mesenchymal stem cells in the treatment of early knee arthritis

目的研究早期膝关节炎患者应用富血小板血浆联合间充质干细胞治疗的临床效果。方法86例早期膝关节炎患者,参照随机抽样法分成对照组和观察组,各43例。对照组实施单纯间充质干细胞治疗,观察组实施富血小板血浆联合间充质干细胞治疗。比较两组患者奎森功能演算指数(Lequesne评分)、视觉模拟评分法(VAS)评分及膝关节液炎症因子[肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-1β与基质金属蛋白酶(MMP)-13]水平。结果治疗1、3个月,观察组Lequesne评分分别为(12.16±2.23)、(9.34±1.75)分,均低于对照组的(14.53±2.28)、(11.67±2.15)分,差异具有统计学意义(P<0.05)。治疗1、3个月,观察组VAS评分分别为(2.80±0.47)、(2.16±0.40)分,均低于对照组的(3.65±0.63)、(2.83±0.43),差异有统计学意义(P<0.05)。治疗前,两组TNF-α、IL-1β与MMP-13水平对比,差异无统计学意义(P>0.05);治疗后,观察组TNF-α、IL-1β与MMP-13水平均低于对照组,差异有统计学意义(P<0.05)。结论富血小板血浆联合间充质干细胞治疗早期膝关节炎,对于改善患者膝关节功能、缓解疼痛等有重要意义。

Objective To study the clinical effect of applying platelet-rich plasma combined with mesenchymal stem cells in the treatment of early knee arthritis.Methods 86 patients with early knee arthritis were randomly divided into control group and observation group,with 43 cases in each group.Patients in the control group were solely treated with mesenchymal stem cells,while patients in the observation group were treated with platelet-rich plasma combined with mesenchymal stem cells.Lequesne score,visual analogue scale(VAS)score and levels of inflammatory factors in knee joint fluid[tumor necrosis factorα(TNF-α),Interleukin(IL)-1βand matrix metalloproteinase(MMP)-13]were compared between the two groups.Results After 1 and 3 months of treatment,the Lequesne scores of the observation group were(12.16±2.23)and(9.34±1.75)points,respectively,which were lower than(14.53±2.28)and(11.67±2.15)points of the control group,and the differences were statistically significant(P<0.05).After 1 and 3 months of treatment,the VAS scores of the observation group were(2.80±0.47)and(2.16±0.40)points,respectively,which were lower than(3.65±0.63),(2.83±0.43)points of the control group,and the differences were statistically significant(P<0.05).Before the treatment,the differences in levels of TNF-α,IL-1βand MMP-13 between the two groups were not statistically significant(P>0.05).After the treatment,levels of TNF-α,IL-1βand MMP-13 of the observation group were lower than those of the control group,and the differences were statistically significant(P<0.05).Conclusion The treatment of early knee arthritis with platelet-rich plasma combined with mesenchymal stem cells is of significant importance in improving knee function and relieving pain of patients.