基于线粒体标记的滁州鲫遗传多样性和遗传结构

Genetic Diversity and Genetic Structure of Chuzhou Crucian Carp based on Mitochondrial Markers

为了解滁州鲫遗传种质资源现状,采用线粒体细胞色素b和控制区为分子标记,研究了滁州鲫保种群体和野生群体的遗传多样性和遗传结构。研究获得了长度分别为990bp的Cyt b部分序列和907bp的控制区全序列。2个群体相比较,保种群体遗传多样性略低于野生群体。总体而言,基于线粒体细胞色素b和控制区均显示滁州鲫的遗传多样性偏低,但2个群体在2种分子标记中均不存在显著的遗传分化;不同序列间比较,2个群体的控制区不仅A+T含量更高,还具有更高的单倍型多样性和核苷酸多样性,表明控制区更适于研究滁州鲫的群体遗传变异。

In order to understand the genetic resources of Chuzhou crucian carp,mitochondrial cytochrome b and control region were used as molecular markers to study the genetic diversity and structure of the conserved and wild crucian carp populations.A partial sequence of 990 bp of Cyt b and a full sequence of 907 bp of control region were obtained.The genetic diversity of the conserved population was slightly lower than that of the wild population,but there was no significant genetic differentiation between the two populations with two molecular markers.In general,the genetic diversity of Chuzhou crucian carp was low with two molecular markers.The comparison between different sequences showed that the control region not only had higher A+T content,but also had higher haplotype diversity and nucleotide diversity,indicating that the control region was more suitable for studying population genetic variation of Chuzhou crucian carp.