利用Connectivity Map数据库筛选抗菌药物的初步研究

The study of antimicrobial drugs screening using Connectivity Map database

为了筛选新的抗菌药物并于体外验证其抗菌活性,利用猪链球菌感染的THP-1细胞基因芯片结果,通过Connectivity map(Cmap)数据库筛选抗菌药物。测定其对大肠杆菌(Escherichia coli)、绿脓杆菌(Pseudomonas aeruginosa)、金黄色葡萄球菌(Staphylicoccus aureus)、猪丹毒杆菌(Erysipelothrix rhusiopathiae)、猪链球菌(Streptococcus suis)的最低抑菌浓度及其对溶血活性的抑制作用,初步筛选潜在抗菌活性的药物。结果表明,Cmap筛选到石蒜碱、左旋水苏碱和玫瑰树碱等3种生物碱具有潜在抗菌活性。体外抗菌试验结果显示玫瑰树碱对大肠杆菌、金黄色葡萄球菌、猪丹毒杆菌、猪链球菌均有显著的抑菌作用,且对金黄色葡萄球菌、猪链球菌的溶血活性有明显的抑制作用。石蒜碱和左旋水苏碱抑菌效果不明显。

In order to screen new antibacterial agents and verify their antibacterial activity in vitro,the antimicrobial agents were screened by Connectivity Map(Cmap)database based on the gene chip results of streptococcus suis infected THP-1 cells.Antibacteri⁃al potential drugs were screened and the minimum inhibitory concentration(MIC)and the inhibitory effect on hemolytic activity of Escherichia coli,Pseudomonas aeruginosa,Staphylicoccus aureus,Erysipelothrix rhusiopathiae,and Streptococcus suis were deter⁃mined in vitro.The results showed that three alkaloids lycorine,betonicine and ellipticine,were screened out by Cmap.The MIC re⁃sults showed that ellipticine had significant inhibitory effect on Escherichia coli,Staphylicoccus aureus,Erysipelothrix rhusiopathiae,and Streptococcus suis.And ellipticine had significant inhibitory effect on hemolytic activity of Staphylococcus aureus and Streptococcus suis.But the antibacterial effect of lycorine and betonicine were not significant.