牙槽骨炎性肉芽组织及修复性肉芽的差异研究

Study on the difference between inflammatory granulation tissue and restorative granulation tissue in alveolar bone

目的:探究牙槽骨炎性肉芽组织(IGT)与修复性肉芽组织(RGT)之间的组织学及生物信息学差异,以期为通过调控IGT向RGT转化进而促进牙槽骨炎性病损治疗提供基础。方法:收集10例接受延期自体牙移植的患者,收集一期牙拔除术中刮除的IGT及二期牙移植术中刮除的RGT。随机抽取3例样本用于HE染色、Masson染色及免疫组织化学染色,另外7例行串联质谱标签标记的定量蛋白质组学分析。结果:与IGT相比,RGT显著特征为大量有序排列且成熟的纤维组织,且成纤维细胞、Osterix阳性细胞等骨形成相关细胞数目明显增多。生物信息学结果提示两者的差异主要分布于细胞外基质及炎症相关通路,COL1A1是二者间表达差异最为显著的分子,亦是差异表达蛋白相互作用调节网络中的一个中枢。COL1A1的主要降解酶——组织蛋白酶K(CTSK)在RGT中的表达显著降低。结论:IGT与RGT存在异同,CTSK有望成为调控IGT向RGT转化的潜在靶点。

Objective:To investigate the difference in histological and bioinformatics analysis between inflammatory granulation tissue(IGT)and restorative granulation tissue(RGT)in alveolar bone in order to provide a basis for the treatment of alveolar bone inflammatory lesions by regulating IGT to RGT transformation.Methods:Ten patients receiving delayed autogenous dental transplantation were collected.IGT and RGT were collected from these patients during primary tooth extraction and secondary tooth transplantation,of which three cases were randomly selected for HE staining,Masson staining and immunohistochemical(IHC)staining,and the other seven cases were quantitatively analyzed by tandem mass spectrometry label.Results:Compared with IGT,RGT was characterized by a large number of well-arranged and mature fibrous tissues,and the number of bone formation-related cells such as fibroblasts,Osterix positive cells were significantly increased.The bioinformatics results suggested that the differences were mainly in the extracellular matrix and inflammation-related pathways.COL1A1 was significantly up-regulated and played a key role in protein-protein interaction regulatory network.As the main degrading enzyme of COL1A1,cathepsin K(CTSK)was significantly down-regulated in RGT.Conclusions:There were both some similarities and differences between IGT and RGT in histology,and CTSK might be a specific target to regulate transformation from IGT to RGT in alveolar bone.