摘要
Background:Gossypium herbaceum(G.herbaceum),a plant commonly found in the wild in Nigeria,is said to possess some therapeutic activities.However,there is a dearth of information on its chemical constituents.Also,there is a need to investigate its therapeutic activities.Objective:To investigate the qualitative and quantitative phytochemical components of the leaf extracts of G.herbaceum,as well as its antioxidant,antimalarial and cytotoxic activity.Methods:Gas chromatography-mass spectrometry(GC-MS)analysis was used to determine the components of ethanol and hexane extracts of G.herbaceum leaf while 2,2-diphenyl-1-picrylhydrazyl(DPPH)and nitric oxide assays were used to determine the antioxidant potential.Malaria parasites viability was examined using parasite lactate dehydrogenase(pLDH)technique and HeLa cell was used for the cytotoxicity evaluation.Results:Bioactive compounds identified in ethanol and hexane extracts of G.herbaceum were 37 and 30 kinds,respectively,with major components as linoleic acid(36.10%and 33.82%),vitamin E(7.15%and 5.98%)and caryophyllene(4.21%and 5.08%).It also has tannins,saponins,alkaloids,flavonoids steroids,phenols and ter-penoids.In antibacterial test,significant inhibitory potentials against multidrug-resistant bacteria strains were present.In vitro antioxidant potentials(IC 50)of ethanol and hexane extracts were 3.33 and 4.12μg/mL for DPPH assays,and 3.87 and 5.00μg/mL for nitric oxide assays,respectively.Antiplasmodial activities(IC 50)were 9.99 and 9.76μg/mL for ethanol and hexane extracts,respectively.Conclusion:G.herbaceum leaf may provide novel plant-derived therapeutic agents,effective in treating infectious diseases arising from multiple drug-resistant bacteria and a target in the management of oxidative stress.
