摘要
目的研究黄芩苷对SD大鼠脑中动脉的影响。方法应用压力型小动脉测量仪观察给予黄芩苷前后脑中动脉直径的变化;应用全细胞膜片钳技术和内面向外式膜片钳技术观察黄芩苷对离体的SD大鼠脑中动脉SMC电流的调节作用。结果黄芩苷舒张SD大鼠脑中动脉血管段呈浓度依赖性。BK通道阻断剂ibTX阻断黄芩苷的舒张作用。黄芩苷增强脑中动脉SMC外向电流呈浓度依赖性。BK通道阻断剂ibTX阻断黄芩苷介导的脑中动脉SMC外向电流。黄芩苷增加BK通道开放频率。结论黄芩苷增加BK通道开放频率,增强其介导的外向电流,舒张SD大鼠脑中动脉血管。
Aim To study the effect of baicalin on middle cerebral artery in SD rats. Methods The changes of middle cerebral artery diameter were observed using a pressure myograph system. The whole-cell and inside-out patch-clamp recording were used to detected the electrophysiological features of single vascular smooth muscle cells. Results Baicalin dilated the middle cerebral artery segment of SD rats in a concentration-dependent manner. IbTX blocked baicalin-mediated relaxation. Baicalin enhanced the outward current of middle cerebral artery smooth muscle cells in a concentration-dependent manner. IbTX blocked baicalin-mediated outward current. Baicalin increased BK channel open probabilities. Conclusions Baicalin enhances the outward current mediated by BK channel and relaxes the middle cerebral artery in SD rats.
作者
王盛
蒋蕾蕾
魏雯
王家宁
张传林
WANG Sheng;JIANG Lei-lei;WEI Wen;WANG Jia-ning;ZHANG Chuan-lin(Pharmacy Dept,The First Affiliated Hospital,Shihezi University Medical College,Shihezi Xinjiang 832000,China;Electrophysiological Laboratory,Laboratory of Xinjiang Endemic and Ethnic Diseases,Shihezi Xinjiang 832002,China;Heart Function Dept,Renmin Hospital,Hubei University of Medicine,Shiyan Hubei 442000,China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2022年第1期43-46,共4页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 81270221)。
