56434例女性人乳头瘤病毒感染与基因亚型分布情况

Infection of human papillomavirus and gene subtypes distribution in 56434 female patients

目的分析江苏省扬州市某医院女性人乳头瘤病毒(HPV)阳性感染情况,为扬州地区HPV防控策略的制订和疫苗的选用提供参考依据。方法选取2018年1月—2020年12月江苏省扬州市妇幼保健院56434例就诊女性的宫颈上皮脱落细胞标本,采用基因扩增及导流杂交技术对37种HPV亚型进行分型检测。结果共检出HPV阳性样本12900份,检出率为22.86%(12900/56434),其中单一感染(以高危型HPV亚型感染为主)占77.35%、多重感染(以高危型HPV亚型感染+低危型HPV亚型感染为主)占22.65%;36种HPV基因亚型被检出,检出率居前5位的高危型HPV亚型为HPV52、HPV16、HPV58、HPV53和HPV51;26~35岁女性的HPV感染检出率最高(25.07%),其次为36~45岁女性(24.45%)和46~55岁女性(20.96%)。结论2018—2020年江苏省扬州市妇幼保健院就诊女性HPV感染以单一型感染、高危型感染为主,其中以HPV52、HPV16、HPV58、HPV53和HPV51基因亚型多见,扬州地区女性预防接种宜选用针对亚型中包含这5种亚型的HPV疫苗。

Objective To investigate the positive infection of human papillomavirus(HPV) in a hospital in Yangzhou of Jiangsu Province, and provide references for HPV prevention and control strategy as well as selection of vaccine. Methods The cervical epithelial cell samples from 56 434 women from January 2018 to December 2020 were selected, and 37 HPV subtypes were tested by gene amplification and diversion hybrid. Results A total of 12 900 HPV positive samples were detected, with a positive rate of 22.86%(12 900/56 434), among which single infection(mainly high-risk HPV subtypes infection) accounted for 77.35%, and multiple infections(mainly high-risk and low-risk HPV subtypes infection) for 22.65%. A total of 36 subtypes of HPV were detected, HPV 52, HPV16, HPV58, HPV53 and HPV51 ranked the top five in detection rate. The detection rate of HPV infection was the highest in women aged 26 to 35(25.07%), followed by women aged 36 to 45(24.45%) and women aged 46 to 55(20.96%). Conclusion HPV positive Females in Yangzhou Maternal and Child Health Care Hospital of Jiangsu Province from 2018 to 2020 are mainly infected by single and high risk subtypes, HPV 52, HPV16, HPV58, HPV53 and HPV 51 gene subtypes are commonly seen. Therefore, HPV vaccines for these 5 subtypes should be selected for women in Yangzhou.