摘要
噬菌体的内溶酶(endolysin)和穿孔素(holin)是新型的抑菌剂,具有很大的潜力和发展空间。该文以大肠杆菌O157∶H7噬菌体EC-p9和沙门氏菌噬菌体SM-p2的内溶酶和穿孔素(Lys 9、Lys 2、Hol 9、Hol 2)为对象,通过平板菌落计数法研究了Lys 9、Lys 2、Hol 9、Hol 2的裂解活性;评估Lys 9、Lys 2、Hol 9、Hol 2对温度和pH的稳定性;研究了细菌是否会对这4种重组蛋白产生抗性;还研究了内溶酶、穿孔素及其混合物对细菌的联合抑菌作用。结果表明,Lys 9、Lys 2、Hol 9和Hol 2的最高裂解活性分别为61.348%、81.120%、74.251%和83.356%,具有很强的杀菌能力;Lys 9、Lys 2、Hol 9和Hol 2对温度和pH有较高的稳定性;12代内细菌不会对Lys 9、Lys 2、Hol 9和Hol 2产生抗性。此外,内溶酶和穿孔素在抑制病原菌上表现出协同作用。该实验结果为噬菌体内溶酶和穿孔素联合抑菌作用提供了理论依据。
The endolysin and holin of phage are new bacteriostatic agents with great potential and prospect.Lysis activity and bacteria resistance for Lys 9,Lys 2,Hol 9 and Hol 2 were studied.The stability of Lys 9,Lys 2,Hol 9 and Hol 2 to temperature and pH were evaluated by plate count using endolysin and holin of Escherichia coli O157:H7 phage EC-P9 and Salmonella phage SM-P2.The combined antibacterial effect of endolysin,holin and their mixtures was also determined.The results showed that Lys 9,Lys 2,Hol 9 and Hol 2 had strong bactericidal ability and the highest lysing activity of bacteria were 61.348%,81.120%,74.251%and 83.356%,respectively.Lys 9,Lys 2,Hol 9 and Hol 2 had excellent stability in a wide range of temperature and pH.The bacteria did not develop resistance to Lys 9,Lys 2,Hol 9 and Hol 2 within 12 generations.In addition,endolysin and holin showed synergistic effect on the inhibition of bacteria.The experimental results provide a theoretical basis for the combined antibacterial effect of endolysin and holin in phage.
作者
史东灵
解天慧
石慧
SHI Dongling;XIE Tianhui;SHI Hui(College of Food Science,Southwest University,Chongqing 400715,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2021年第24期7-12,共6页
Food and Fermentation Industries
基金
国家自然科学基金面上项目(32072322)
重庆市技术创新与应用发展项目面上项目(cstc2019jscx-msxm1593)
重庆市留学人员创业创新计划优秀项目(cx201907)。
关键词
噬菌体
内溶酶
穿孔素
特性
联合抑菌
phage
endolysin
holin
features
combined bacteriostasis