漠河蓝果忍冬多酚成分鉴定及其体外生物活性分析

Identification and in vitro biological activity analysis of polyphenolic from Mohe blue honeysuckle

该文以漠河蓝果忍冬果实为研究对象,采用高效液相色谱-电喷雾质谱对其花色苷和非花色苷多酚的组分进行分离鉴定,并且分析其抗氧化活性、抗脂肪酶活性、抗α-淀粉酶活性及抗α-葡萄糖苷酶活性。结果表明,漠河市蓝果忍冬果实中含有8种花色苷,其中矢车菊-3-葡萄糖苷所占总和的比例最大,而24种非花色苷多酚中5-咖啡酰奎宁酸含量最高。漠河蓝果忍冬总酚含量高达(108.52±6.80)mg GAE/100g DW,总花青素含量为(23.36±0.54)mg C3G/100g DW。在抗氧化方面具有较强的生物活性:清除DPPH自由基的能力为(201.19±9.97)μmol TE/g DW、铁离子还原能力为(5.16±0.16)μmol TE/g DW、总抗氧化能力为(658.18±9.73)μmol TE/g DW、抗氧化能力为(414.79±2.08)μmol TE/g DW,并且在抑制碳水化合物水解酶(抑制α-淀粉酶的IC_(50)值为6.399 mg/mL、抑制α-葡萄糖苷酶的IC_(50)值为11.73 mg/mL)和脂肪酶(IC_(50)值为1.912 mg/mL)方面也表现出一定的生物活性。

In this study,the anthocyanins and non anthocyanin polyphenols of Mohe blue honeysuckle were isolated and identified by high performance liquid chromatography-electrospray tandem mass spectrometry(HPLC-ESI-MS^(2)),and their antioxidant activity,lipase inhibition activity,α-amylase inhibition activity,andα-glucosidase inhibition activity were analyzed.The results showed that blue honeysuckle fruits in Mohe contained 8 kinds of anthocyanins,among which the proportion of cyanidin-3-O-glucoside was significantly high,and the content of 5-caffeoylquinic acid was the highest among 24 kinds of non-anthocyanins.The contents of the total phenolics and the total anthocyanins in crude extracts of blue honeysuckle fruits in Mohe reached(108.52±6.80)mg GAE/100g DW and(23.36±0.54)mg C3G/100g DW,which showed strong antioxidant activity(201.19±9.97)μmol TE/g DW,(5.16±0.16)μmol TE/g DW,(658.18±9.73)μmol TE/g DW,and(414.79±2.08)μmol TE/g DW against DPPH,FRAP,ABTS,and ORAC assays,respectively.The results also showed certain biological activity of the inhibition activity ofα-amylase,α-glucosidase and lipase.The semi-inhibitory concentrations ofα-amylase,α-glucosidase and lipase were 6.399 mg/mL,11.73 mg/mL and 1.912 mg/mL,respectively.The results of this study provides scientific references and valuable insights for blue honeysuckle fruit in Mohe.