酒精性肝病患者血清IL-17水平及其基因-197A/G位点单核苷酸多态性分析

Serum IL-17 levels and single nucleotide polymorphism of its gene at-197A/G locus in patients with alcoholic liver diseases

目的探讨酒精性肝病(ALD)患者血清白细胞介素-17(IL-17)水平及其基因-197A/G位点单核苷酸多态性的变化。方法2015年10月~2020年10月我院收治的酒精性脂肪肝患者44例、酒精性肝炎患者40例、酒精性肝硬化患者34例和ALD相关性肝癌患者18例,采用ELISA法检测血清IL-17水平,采用单核苷酸检测试剂盒检测外周血IL-17-197A/G位点基因多态性。结果肝癌患者血清IL-17水平为(8.7±1.4)pg/mL,显著高于酒精性脂肪肝、酒精性肝炎和酒精性肝硬化患者【分别为(3.7±0.3)pg/mL、(4.0±0.6)pg/mL和(6.9±0.8)pg/mL,均P<0.05】;肝癌患者血清ALT、AST、GGT和ALB水平分别为(54.3±13.3)U/L、(53.8±13.7)U/L、(262.2±17.9)U/L和(33.9±13.8)g/L,酒精性肝硬化患者分别为(39.8±8.8)U/L、(40.1±7.2)U/L、(251.1±7.9)U/L和(31.1±2.6)g/L,酒精性肝炎患者分别为(84.0±7.5)U/L、(75.4±6.8)U/L、(245.1±5.6)U/L和(43.1±5.7)g/L,与酒精性脂肪肝患者【分别为(29.5±5.2)U/L、(30.5±7.8)U/L、(142.7±5.2)U/L和(42.7±3.2)g/L,均P<0.05】比,差异显著;肝癌和酒精性肝硬化患者AA基因型分布频率分别为77.9%和64.7%,显著高于酒精性脂肪肝和酒精性肝炎患者(分别为22.7%和27.5%,均P<0.05),A等位基因分布频率分别为83.3%和75.0%,显著高于酒精性脂肪肝和酒精性肝炎患者(分别为26.1%和33.8%,均P<0.05)。结论高水平的血清IL-17可能与ALD病情进展相关,外周血IL-17-197A/G位点AA基因型和A等位基因是ALD发生的易感基因,可能增加酒精性肝硬化甚至肝癌发生的风险。

Objective The aim of this study was to investigate serum interleukin-17(IL-17)levels and the single nucleotide polymorphism(SNP)of its gene at-197A/G locus in patients with alcoholic liver diseases(ALD).Methods 44 patients with alcoholic fatty liver,40 with alcoholic hepatitis,34 with alcoholic cirrhosis and 18 with ALD-related liver cancer were recruited in this study between October 2015 and October 2020,and serum IL-17 level was detected by ELISA.The gene polymorphisms at-197A/G locus were detected by single nucleotide test kits.Results Serum IL-17 level in patients with liver cancer was(8.7±1.4)pg/mL,significantly higher than[(3.7±0.3)pg/mL,(4.0±0.6)pg/mL and(6.9±0.8)pg/mL,respectively,P<0.05]in patients with alcoholic fatty liver,alcoholic hepatitis and alcoholic cirrhosis;serum ALT,AST,GGT and ALB levels in patients with liver cancer were(54.3±13.3)U/L,(53.8±13.7)U/L,(262.2±17.9)U/L and(33.9±13.8)g/L,those in patients with alcoholic liver cirrhosis were(39.8±8.8)U/L,(40.1±7.2)U/L,(251.1±7.9)U/L and(31.1±2.6)g/L,in alcoholic hepatitis were(84.0±7.5)U/L,(75.4±6.8)U/L,(245.1±5.6)U/L and(43.1±5.7)g/L,all significantly different compared to[(29.5±5.2)U/L,(30.5±7.8)U/L,(142.7±5.2)U/L and(42.7±3.2)g/L,respectively,P<0.05]in patients with alcoholic fatty liver;the distribution frequencies of AA genotype of IL-17 gene at-197A/G locus in patients with liver cancer and alcoholic cirrhosis were 77.9%and 64.7%,significantly higher than 22.7%and 27.5%(P<0.05)in patients with alcoholic fatty liver and alcoholic hepatitis,and the distribution frequencies of A allele in patients with liver cancer and alcoholic cirrhosis were 83.3%and 75.0%,significantly higher than 26.1%and 33.8%(P<0.05)in patients with alcoholic fatty liver and alcoholic hepatitis.Conclusion High serum level of IL-17 might be related with the progression of ALDs and the AA genotype and A allele of IL-17 at-197A/G locus could be the susceptibility genes of patients with ALD,which might increase the risk of alcoholic liver cirrhosis and even liver cancer.