摘要
In order to study how exogenous hormones in C.lanceolata(gymnosperm)regulate somatic embryogenesis,we measured the endogenous phytohormones of two genotypes with different somatic embryogenesis efficiency and found that an increase in endogenous concentrations of IAA and ABA may be correlated to more efficient somatic embryogenesis.By applying exogenous spermidine,we found that exogenous hormones may affect somatic embryogenesis efficiency through affecting the endogenous phytohormone content.Based on these results,further studies can be conducted whereby the concentration of exogenous hormones or the levels of endogenous phytohormones by molecular methods are regulated to promote somatic embryogenesis.Our research may benefit the long-term economic output of the forestry industry and lays the foundation to studying the molecular mechanism that controls somatic embryogenesis efficiency.
基金
This research was supported by Foundation of Jiangsu forestry bureau(LYKJ[2017]42)
Key research and development plan of Jiangsu Province(BE2017376)
the Nature Science Foundation of China(31770715)
the Qinglan project of Jiangsu province,Priority Academic Program Development of Jiangsu Higher Education Institutions,Natural Science Foundation of Jiangsu Province(BK20181176)
the Joint Fund of the Natural Science Foundation of China and the Karst Science Research Center of Guizhou Province(Grant No.U1812401).