肠型胃腺癌组织中A3G的表达及其临床病理学意义

Expression of A3G in gastric adenocarcinoma and its clinical significance

目的探讨胃腺癌组织中A3G的表达及其与临床病理特征和预后的关系。方法通过在线工具GEPIA分析胃癌组织与癌旁组织中A3G mRNA的表达差异。收集胃腺癌(Lauren分型肠型)组织样本85例,应用免疫组化EliVision两步法检测胃癌组织中A3G蛋白的表达,并分析其与临床病理特征及肿瘤浸润淋巴细胞数量的关系。通过TIMER数据库分析胃癌组织中A3G mRNA表达与肿瘤浸润淋巴细胞(B细胞、CD4^(+)T细胞、CD8^(+)T细胞)的关系。结果胃癌组织中A3G mRNA表达水平高于癌旁组织。A3G蛋白低表达42例(49.4%),高表达43例(50.6%)。胃癌组织中A3G蛋白高表达与肿瘤T、N分期、临床分期及肿瘤浸润淋巴细胞数量相关(P<0.05),A3G高表达的病例肿瘤浸润更深、淋巴结转移更多、分期更晚;A3G蛋白表达与患者性别、年龄、肿瘤部位、远处转移等因素无关(P>0.05)。A3G高、低表达患者的5年总生存率分别为46.5%、71.4%,差异有统计学意义(P<0.05)。胃癌组织中A3G表达与肿瘤浸润T细胞呈正相关(r=0.668,P<0.05)。结论A3G在胃腺癌中高表达,与肿瘤侵袭转移临床病理特征和肿瘤免疫微环境密切相关,其可能参与胃腺癌的发生、发展,可作为胃腺癌预后标志物或潜在的治疗靶点。

Purpose To investigate the expression of A3G in gastric adenocarcinoma,and to analyze its correlation with clinicopathologic parameters and prognosis.Methods The difference of A3G mRNA expression between gastric cancer tissues and adjacent tissues was analyzed by online tool GEPIA.85 cases of gastric adenocarcinoma(Lauren’s intestinal type)were collected.The expression of A3G protein in gastric cancer was detected by immunohistochemistry,and its relationship with clinicopathological features and tumor infiltrating lymphocytes were analyzed.The relationship between A3G mRNA expression and tumor infiltrating lymphocytes(B cells,CD4^(+)T cells,CD8^(+)T cells)in gastric cancer was analyzed by TIMER database.Results The expression level of A3G gene in gastric cancer tissues was higher than that in adjacent tissues(P<0.05).A3G low expression was 42 cases(49.4%),A3G high expression was 43 cases(50.6%).The high expression of A3G protein in gastric cancer was correlated with T,N,clinical stages and tumor infiltrating lymphocytes(P<0.05).The gastric cancers with high expression of A3G had deeper tumor invasion,more lymph node metastasis and later clinical stage.The expression of A3G protein was not related to gender,age,tumor location and distant metastasis(P>0.05).The 5-year overall survival rate of patients with high expression of A3G was lower than that of patients with low expression of A3G,the 5-year overall survival rates were 46.5% and 71.4% respectively,and the difference was statistically significant(P<0.05).The expression of A3G in gastric cancer was positively correlated with tumor infiltrating CD8^(+)T cells(r=0.668,P<0.05).Conclusion The overexpression of A3G in gastric adenocarcinoma(Lauren’s intestinal type)and is significant correlation with the clinicopathological parameters of tumor invasion and metastasis and the tumor immune microenvironment.A3G may be involved in the development and progression of gastric adenocarcinoma and may be a potential prognostic marker or new therapeutic target for gastric cancer in the future.