摘要
The need for co-ordinate,high-level,and stable expression of multiple genes is essential for the engineering of biosynthetic circuits and metabolic pathways.This work outlines the functionality and design of IRES-and 2 A-peptide-based constructs by comparing different strategies for co-expression in polycistronic vectors.In particular,2 A sequences are small peptides,mostly derived from viral polyproteins,that mediate a ribosome-skipping event such that several,different,separate proteins can be generated from a single open reading frame.When applied to metabolic engineering and synthetic gene circuits,2 A peptides permit to achieve co-regulated and reliable expression of various genes in eukaryotic cells.
