质谱分析导向的稳定同位素标记技术进展

Recent Progress on Mass Spectrometry Analysis-oriented Stable Isotopic Labeling Technologies

介绍了近年来发展的一系列具有代表性的质谱分析导向的低残留稳定同位素标记技术:二甲氧基甲砜基嘧啶衍生化、低残留稳定同位素标记季铵化衍生化、新型胍剂化合物的衍生化等技术与方法。一些在常规电喷雾或基质辅助激光解吸电离条件下无法有效离子化的目标分子,在衍生化后产生新的正电荷中心或成为容易被电离物种而被质谱检测,从而提高了这类化合物的质谱学检测灵敏度。该类研究显著优势在于所开发的衍生化试剂本身不是离子型化合物,新产生的电荷中心或易于离子化的基团由衍生化反应引入。过量的衍生化试剂易于除去,不会对随后的质谱分析产生明显干扰。这一系列研究成果在代谢组学、食品安全分析、质谱成像以及单细胞分析等方面具有广泛的应用价值。

This article introduced a series of representative mass spectrometry-oriented low-residual stable isotope labeling techniques developed in recent years:dimethoxy-methylsulfoxide pyrimidine-based derivatization,low residual stable isotopic labeling quaternary ammonium derivatization,and new guanidino compounds derivatization and other technologies and methods.Derivatization reactions introduced isotope labeling charge tags or easily-ionized groups into the target molecules for detected by mass spectrometry,which could not be effectively ionized under conventional ESI or MALDI conditions.Thus,the sensitivity of mass spectrometry detection was improved of these compounds.The most significant advantage of these researches is that the developed derivatization reagent were not ionic compounds,and the newly isotope labeling charge tags or easily-ionized group is introduced by the derivation biochemical reaction.Excessive derivatization reagents were easy to remove and will not significantly interfere with subsequent mass spectrometry analysis.Therefore,these derivatization reagents and methods would have wide range of potential applications in metabolomics,food safety analysis,mass spectrometry imaging and single cell analysis.