一种制备唾液酸磁性表面分子印迹聚合物的新方法

A Novel Method for Preparing of Sialic Acid Magnetic Surface Molecularly Imprinted Polymer

一锅法合成氨基化磁性纳米颗粒Fe_(3)O_(4)@NH_(2),与对甲酰基苯硼酸(FPBA)反应嫁接硼酸官能团,通过硼酸基与模板唾液酸Neu5Ac分子上的顺式二醇共价反应,将Neu5Ac定向固定于磁性纳米颗粒。以多巴胺(DA)及3-氨基苯硼酸(3-APBA)为功能单体,自聚合反应形成共聚壳层包覆在磁性纳米颗粒的表面,制备得Neu5Ac磁性分子印迹聚合物(Neu5Ac-MMIPs)。通过透射电镜、红外光谱对其形态及结构进行表征,并评价其吸附性能。结果表明,Neu5Ac磁性分子印迹聚合物对Neu5Ac具有较好的吸附量、印迹效率、特异性等优点。通过对Neu5Ac至少5次吸附-洗脱的循环实验表明,MMIPs具有较好的重复再利用能力。

Amino-functionalized magnetic nanoparticles Fe_(3)O_(4)@NH_(2) were synthesized by one-pot method,then grafted with boric acid group on their surface by reaction with p-formyl phenylboric acid(FPBA).Templatesialic acid Neu5Ac was directionally fixed to magnetic nanoparticles by covalent reaction between its cis-diol group and boric acid group.Neu5Ac magnetic molecularly imprinted polymers(Neu5Ac-MMIPs)was prepared by self-polymerization of functional monomers dopamine(DA)and 3-aminophenylboric acid(3-APBA),forming a polymer shell coated on the surface of magnetic nanoparticles.Their morphology and structure were characterized by transmission electron microscopy,infrared spectroscopy,and their adsorption properties were evaluated.The results showed that Neu5Ac-MMIPs had good adsorption capacity,imprinting efficiency and specificity for Neu5Ac.After at least five rounds of adsorption-elution cycling experiments on Neu5Ac,MMIPs still had good reusability.