藏药珊瑚七十味丸对高脂血症模型大鼠的降脂作用及机制研究

Study on effects and mechanism of Tibetan medicine Shanhu qishiwei pill in lowering blood lipid of hyperlipidemia model rats

目的研究藏药珊瑚七十味丸对高脂血症(HLP)模型大鼠的降脂作用,并初步探讨其作用机制。方法将60只SD大鼠按体质量随机分为正常组、模型组、辛伐他汀组(阳性对照,20 mg/kg)和珊瑚七十味丸低、中、高剂量组(50、100、200 mg/kg),每组10只。正常组大鼠饲喂常规饲料,其余各组大鼠饲喂高脂饲料建立HLP模型,连续喂养4周。各给药组大鼠在造模同时即开始灌胃相应药物,正常组和模型组大鼠灌胃等体积生理盐水,每天1次,连续4周。末次灌胃后,检测各组大鼠血清中总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平,观察各组大鼠肝组织病理变化,测定各组大鼠肝组织中AMP活化蛋白激酶1(AMPK)、磷酸化AMPK(p-AMPK)、肝激酶B1(LKB1)、3-羟基-3-甲基戊二酸单酰辅酶A还原酶(HMGCR)蛋白的表达情况。结果低、中、高剂量珊瑚七十味丸均可显著降低HLP模型大鼠血清中TC、TG、LDL-C水平以及肝组织中HMGCR蛋白表达水平(P<0.05),显著升高HLP模型大鼠血清中HDL-C水平及肝组织中AMPK磷酸化水平、LKB1蛋白表达水平(P<0.05),不同程度地改善HLP模型大鼠肝组织的病理变化。结论珊瑚七十味丸能降低HLP模型大鼠的血脂水平;其机制可能与抑制LKB1/AMPK信号通路的传导,从而调节脂代谢相关。

OBJECTIVE To study the effects of Tibetan medicine Shanhu qishiwei pill in lowering blood lipid of hyperlipidemia(HLP)model rats,and to explore its mechanism primarily.METHODS According to their body weigh,60 SD rats were randomly divide into normal group,model group,simvastatin group(positive control,20 mg/kg)and Shanhu qishiwei pill low-dose,medium-dose and high-dose groups(50,100,200 mg/kg),with 10 rats in each group.Normal group was given conventional diet,and other groups were given high-lipid diet to induce HLP model,for consecutive 4 weeks.Administration groups were given relevant medicine intragastrically at the same time of modeling;normal group and model group were given equal volume of normal saline intragastrically,once a day,for consecutive 4 weeks.After last administration,the serum levels of TC,TG,LDL-C and HDL-C were determined;pathological changes of liver tissue were observed;the protein expressions of AMPK,p-AMPK,LKB1,and HMGCR in liver tissue were detected in each group.RESULTS Low-dose,medium-dose and high-dose of Shanhu qishiwei pill could significantly reduce the serum levels of TC,TG and LDL-C and protein expression of HMGCR in liver tissue(P<0.05),while significantly increased serum level of HDL-C,phosphorylation level of AMPK,protein expression of LKB1 in liver tissue in HLP model rats(P<0.05);the pathological changes of liver tissue in HLP model rats were improved to different extents.CONCLUSIONS Shanhu qishiwei pill can reduce the blood lipid level of HLP model rats,and its mechanism may be related to inhibiting the transmission of LKB1/AMPK signal pathway and regulating lipid metabolism.