摘要
Dear Editor,De novo generation of negative-stranded RNA viruses(NSVs)requires efficient transcription of integral viral RNAs with precise termini from cloned plasmids.Studies during the past 25 years with animal NSVs have established the bacteriophage T7 RNA polymerase(Pol)-and endogenous Pol I-based transcription systems as the most efficient platforms for recovery of recombinant NSVs(Bridgen 2012).Unfortunately.
基金
supported by National Natural Science Foundation of China(Grant No.31671996)
Natural Science Foundation of Zhejiang Province,China(Grant No.LZ20C140004)
National Key R&D Program of China(No.2019YFD1001800)。
