生物发光成像技术在肿瘤细胞活体可视化研究中的应用研究进展

Application of bioluminescence imaging technology in visualization of cancer cells in vivo

在肿瘤研究中,以小鼠和斑马鱼为主的动物模型实验作为介于体外细胞实验和临床试验之间重要的中间环节,往往很受青睐。近年来,挑选一些在肿瘤细胞进程中有特殊活性的启动子,构建这类启动子调控的荧光素酶报告基因,或者将荧光素酶基因的cDNA插入到肿瘤特异性表达的基因调控序列中,建立转基因小鼠和斑马鱼动物模型,随后通过生物发光成像技术(BLI),可以在活体动物模型中实现肿瘤细胞进程的可视化研究,包括对肿瘤细胞增殖、凋亡、迁移和免疫反应的实时监控。现综述利用BLI技术和活体动物成像模型在实现肿瘤细胞进程的可视化和确定肿瘤早期发展阶段方面的研究进展,及其用于不同组织器官间活动和肿瘤治疗方面的发展和应用。

As an important intermediate link between in vitro cell experiments and clinical experiments,animal models such as mice and zebrafish models are often preferred in cancer research.In recent years,some promoters with special activity in tumor progression have been selected to construct luciferase reporter genes.Moreover,to establish the transgenic mouse and zebrafish animal models,the cDNA of luciferase gene has been inserted into the regulation sequence of genes which have extra expression in cancer.Subsequently,through Bioluminescence Imaging(BLI),it makes it possible to visualization of tumor progression in vivo in living animal models,including real-time monitoring of cancer cell proliferation,apoptosis,migration and immune response.In this review,we summarize the advances in the use of BLI technology and in vivo animal imaging models to visualize cancer cell and identify early stages of tumor progression,as well as the development and application of BLI technology in the movement of different tissues and organs and cancer therapy.