浑浊型饲用微生态菌剂悬浮剂的制备及稳定性研究

Preparation of Turbid Microbial Suspension Agent for Feed Use and Its Stability Evaluation

[目的]建立一种浑浊型微生态菌剂悬浮剂的制备方法,并对其稳定性进行评价。[方法]以黄原胶和羧甲基纤维素钠为稳定剂,以饲用微生态菌剂干粉为悬浮成分,制备微生物水悬浮体系。对制备的悬浮剂进行稳定性评价,测定指标包括稳定系数、黏度、存放稳定性、冷贮存稳定性和活菌总数。[结果]随着稳定剂添加比例的增加,悬浮剂的稳定系数逐渐增加。添加0.20%稳定剂,体系的稳定系数达到91.38%,极显著(P<0.01)高于空白对照组。悬浮体系的黏度随着稳定剂添加量的增加和贮存温度的降低而增大,当稳定剂添加比例为0.20%时,体系不出现分层现象,达到稳定状态。经过24 h冷贮存(4℃)后,添加0.10%稳定剂的悬浮剂活菌数出现下降,添加0.10%~0.25%稳定剂显著(P<0.05)低于空白对照组,但仍维持在10^(9) CFU/mL以上。[结论]稳定剂添加量为0.20%时,饲用微生态菌剂悬浮剂无分层现象出现,无论常温还是冷贮存24 h后,制成的悬浮剂中活菌数均保持在10^(9) CFU/mL以上,能够保证饲用微生态菌剂的有效活菌数。

[Objective]To establish a method for preparing turbid microbial suspension agent and to evaluate its stability.[Method]Microbial water suspension system was prepared with xanthan gum and sodium carboxymethyl cellulose as stabilizers and microbial feed additive powder agent as suspension components.The stability of the prepared suspension agent was evaluated using indexes including stability coefficient,viscosity,storage stability,cold storage stability,and total number of viable bacteria.[Result]With the increase of stabilizer proportion,the stability coefficient of suspension agent increased gradually.When 0.20%stabilizer was added,the stability coefficient of the system rose to 91.38%which was significantly(P<0.01)higher than the control.The viscosity of the suspension system elevated with the increase of stabilizer addition amount and the decrease of storage temperature.When the stabilizer addition ratio was 0.20%,the system had no stratification and reached a stable state.After 24 hours of cold storage at 4℃,the number of viable bacteria in the suspension agent with 0.10%stabilizer declined.With 0.10%-0.25%stabilizer addition,the number of viable bacteria was significantly(P<0.05)lower than the control,but remained above 10^(9) CFU/mL.[Conclusion]There is no stratification in the prepared turbid microbial suspension agent for feed use when the addition amount of stabilizer is 0.20%.The number of viable bacteria in the prepared suspension agent remains above 10^(9) CFU/mL after 24 hours of cold or room temperature storage,ensuring the effective amount of viable bacteria in the microbial agent for feed use.