数字PCR技术在动物疫病检测中的应用

Application of Digital PCR Assay in Detection of Animal Epidemic Diseases

为了支持动物疫病流行地区的控制及消除计划,以及在动物疫病非流行地区进行有效筛查,必须使用更加准确灵敏的诊断手段。数字PCR可实现绝对定量及痕量核酸的检测,与传统检测方法相比具有不依赖参考物或标准品、对抑制剂具有较高耐受性、灵敏度和精准度更高的优点。数字PCR作为一种定量分析的核酸检测新方法,在动物疫病检测中得到了应用,围绕数字PCR技术的原理、应用、存在的问题及发展趋势等进行综述及分析,为数字PCR在动物疫病检测中的进一步应用提供参考。

In order to support the control and elimination plan in areas where animal epidemic diseases are prevalent,and to conduct effective screening in areas where animal epidemic diseases are not prevalent,more accurate and sensitive diagnostic methods are expected be used.Digital PCR assay can be used in absolute quantification and detection of trace amounts of nucleic acids.Compared with the traditional detection methods,it does not rely on reference substances or standard products,shows higher tolerance to inhibitors,and has higher sensitivity and accuracy.As a novel tool for quantitative analysis of nucleic acid,digital PCR assay has recently been employed in the detection of animal epidemic diseases.This article summarizes and analyzes the principles,applications,existing problems,and developmental trends of digital PCR assay,in hoping to provide references for its further applications in detection of animal epidemic diseases.