睡莲叶片胎生发育转录组分析

Transcriptome Sequencing Analysis of Leaf Vivipary in Water Lily

睡莲叶片胎生现象是繁育途径的重要补充,对种群的传播、扩散和生态环境的适应性有重要作用。通过转录组测序技术筛选和分析睡莲叶片胎生现象相关的代谢路径和调控基因,为深入认识睡莲叶片胎生发育的分子机制提供参考。以叶片具有胎生现象的‘小花睡莲’(X)和叶片无胎生现象的‘蓝星睡莲’(L)为材料,利用RNA-Seq技术对叶片4个发育阶段的叶脐部分进行生物信息学分析。分析对照(L)和样品(X)叶片不同发育阶段测序结果:筛选出的差异表达基因(DEGs)中,34909个基因(48.65%)表达上调,36850个基因(51.35%)表达下调。DEGs分析显示,随着叶片的发育,X和L上调基因和下调基因数均呈增加趋势。对L1-vs-X1、L4-vs-X4阶段的GO和KEGG功能富集分析表明,DEGs主要富集在质膜和膜相关成分、胞外区域、细胞壁等相关的细胞组分中,涉及到代谢过程、生物合成和应急响应等;Pathway代谢通路表明,DEGs主要参与到植物激素信号转导、苯丙烷类生物合成、氨基酸类代谢、类黄酮生物合成、甘油磷脂类代谢以及细胞周期相关等过程。对DEGs进一步分析,克隆出了4个可能参与睡莲叶片胎生发育的转录因子。

Leaf vivipary in water lily(Nymphaea)is an important supplement to breeding,which plays an important role in the propagation and adaptability of species.Transcriptome sequencing technology was used to screen and explore genome-wide analysis of regulatory genes and metabolic pathways involved in leaf vivipary in water lily.This study would lay a foundation for further understanding the molecular mechanism of leaf viviparous development.Viviparous N.micrantha(X)and non-viviparous N.colorata(L)were selected as the experimental materials.By using RNA-Seq technology,four stages of leaf development of the leaf stalk and stem and stem join were tested and analyzed using a series of bioinformatics analysis.The result showed that 34909(48.65%)DEGs were up-regulated and 36850(51.35%)DEGs were down-regulated.DEGs analysis showed that up-regulated genes and down-regulated genes both increased with the leaves development of N.micrantha amd N.colorata.GO and KEGG enrichment analysis of L1-vs-X1 and L4-vs-X4 stage showed that DEGs were mainly enriched in plasmalemma,cytomembrane and extracellular domain cytoderm associated with metabolic process,biosynthesis and stimulus response.Pathway metabolism pathways indicated that DEGs were mainly involved in plant hormone signal transduction,phenylpropanoid biosynthesis,amino acids metabolism,flavonoid biosynthesis,glycerolipid metabolism and cell cycle and other processes.Four transcription factors potentially involved in leaf viviparous development of water lily were cloned based on DEGs analysis.