GPSM1在结直肠癌中的表达及意义

EXPRESSION AND SIGNIFICANCE OF G-PROTEIN SIGNALING MODULATOR 1 IN COLORECTAL CANCER

目的探讨G蛋白信号调节蛋白1(GPSM1)在结直肠癌中的表达及意义。方法收集2015年4月—2016年4月青岛大学附属医院收治的150例原发性结直肠癌病人的石蜡组织样本及临床病理资料。采用免疫组织化学法检测GPSM1在结直肠癌组织及癌旁组织中的表达,采用实时荧光定量PCR法检测结直肠癌病人石蜡组织样本中KRAS和NRAS的突变情况;分析GPSM1在结直肠癌组织中表达水平与病人临床病理特征及预后的相关性。结果结直肠癌组织中GPSM1的表达水平明显高于癌旁组织(χ^(2)=102.938,P<0.001)。GPSM1在结直肠癌组织中的高表达与淋巴结转移(χ^(2)=5.429,P=0.020)及KRAS突变(χ^(2)=4.030,P=0.045)相关。生存分析结果显示,GPSM1高表达组结直肠癌病人的无病和总体生存期较GPSM1低表达组明显缩短(P=0.046、0.036)。结论GPSM1在结直肠癌组织中高表达,并且与病人不良预后相关,可作为预测结直肠癌转移的潜在生物学指标。

Objective To investigate the expression and significance of G-protein signaling modulator 1(GPSM1)in colorectal cancer(CRC).Methods Paraffin-embedded tumor specimens and clinicopathological data were collected from 150 patients with primary CRC who were admitted to The Affiliated Hospital of Qingdao University from April 2015 to April 2016.Immunohistochemistry was used to measure the expression of GPSM1 in CRC tissue and adjacent tissue,and quantitative real-time PCR was used to measure KRAS and NRAS mutations in paraffin-embedded tumor specimens.The association of GPSM1 expression in CRC tissue with clinicopathological features and prognosis was analyzed.Results The expression level of GPSM1 in CRC tissue was significantly higher than that in the corresponding adjacent tissue(χ^(2)=102.938,P<0.001).The high expression of GPSM1 in CRC tissue was significantly associated with lymph node metastasis(χ^(2)=5.429,P=0.020)and KRAS mutation(χ^(2)=4.030,P=0.045).The survival analysis showed that compared with the low GPSM1 expression group,the high GPSM1 expression group had significantly shorter disease-free survival time and overall survival time(P=0.046,0.036).Conclusion GPSM is highly expressed in CRC tissue,which might be associated with the poor prognosis of CRC patients,and GPSM1 may be used as a potential biological marker for predicting the metastasis of CRC.