淫羊藿多糖对淫羊藿苷和宝藿苷Ⅰ溶解的影响及机制研究

Effects and mechanism of epimedium polysaccharide on solubility of icariin and baohuosideⅠ

通过评价淫羊藿多糖溶液对难溶性黄酮成分淫羊藿苷和宝藿苷Ⅰ溶解度的影响,对淫羊藿多糖的增溶功能及机制进行初步探索。应用高效液相色谱法比较淫羊藿苷和宝藿苷Ⅰ在水中和多糖溶液中的溶解度,并通过差示扫描量热法和临界胶束浓度的测定,研究淫羊藿多糖对淫羊藿苷和宝藿苷Ⅰ溶解度的影响和作用机制。结果表明,淫羊藿粗多糖对淫羊藿苷和宝藿苷Ⅰ的增溶作用具有浓度依赖性。淫羊藿苷和宝藿苷Ⅰ在20 mg·mL^(-1)淫羊藿多糖EPS-1-1的溶解度是水中的9.05、5.76倍;而在20 mg·mL^(-1)淫羊藿多糖EPS-2-1中的溶解度则是水中的10.55、8.39倍。化合物热力学曲线图的改变说明淫羊藿苷、宝藿苷Ⅰ与多糖形成了新的复合物;临界胶束浓度测定表明EPS-1-1和EPS-2-1均具有胶束性质。因此淫羊藿多糖能够显著增加淫羊藿苷、宝藿苷Ⅰ的溶解度,其机制可能和淫羊藿多糖与难溶性黄酮组分形成胶束形式的复合物有关。

This study evaluated the effects of epimedium polysaccharide(EPS)on the solubility of icariin and baohuosideⅠso as to preliminary explore its solubilization function and the underlying mechanism.The solubility of these two insoluble flavonoids in water and polysaccharide solutions was compared by high performance liquid chromatography,and the mechanism was investigated by differential scanning calorimetry(DSC)and critical micelle concentration determination.The results indicated that their solubilization in crude EPS solutions was concentration-dependent.The solubility of icariin and baohuosideⅠin 20 mg·mL^(-1) EPS-1-1 was 9.05 times and 5.76 times that in water,respectively;while their solubility in 20 mg·mL^(-1) EPS-2-1 was 10.55 and 8.39 times that in water,respectively.The change of the DSC thermograms suggested the formation of new complexes from icariin and baohuosideⅠwith polysaccharides.The critical micelle concentrations proved the micellar properties of both EPS-1-1 and EPS-2-1.In short,EPS can significantly increase the solubility of icariin and baohuosideⅠ,the mechanism of which may be related to the formation of micellar complexes between EPS and insoluble flavonoids.