盐炙对沙苑子中沙苑子苷A、B在大鼠体内的药代动力学影响研究

Complanatoside A and complanatoside B in rats administered with raw and salt-processed Astragali Complanati Semen:a pharmacokinetics study

该文建立了UPLC-MS/MS同时测定大鼠体内沙苑子苷A和沙苑子苷B的含量测定方法,并用于研究炮制对这2个成分在大鼠体内的药代动力学影响。以芦丁为内标,采用UPLC-MS/MS同时测定大鼠血浆中沙苑子苷A和沙苑子苷B的含量,运用DAS 3.2.6软件模拟药代动力学过程,计算药代动力学参数,并用SPSS 23.0软件对主要参数进行t检验。结果表明,盐炙前后,生品组沙苑子苷B达峰时间较盐炙组无显著差异,但盐炙组峰浓度和药时曲线下面积AUC_(0-12 h)较生品组升高,并具有显著性差异,平均驻留时间MRT_(0-12h)分别由(3.34±0.44)h缩短为(1.81±0.36)h,差异具有统计学意义(P<0.05)。沙苑子苷A在生品组峰质量浓度为(14.72±11.13)μg·L^(-1),平均滞留时间为(3.93±0.26)h,盐炙品组中峰质量浓度为(35.64±21.99)μg·L^(-1),平均滞留时间缩短为(1.43±0.24)h,差异均具有统计学意义(P<0.05)。因此,可以推断沙苑子盐炙后,能促进沙苑子苷A和沙苑子苷B的体内吸收,并加快排泄。

An UPLC-MS/MS method was developed to simultaneously determine complanatoside A and complanatoside B in rat plasma with rutin as the internal standard and applied to examine the effect of salt-processing on pharmacokinetics of these two flavonoid glycosides.The pharmacokinetic parameters were estimated using DAS 3.2.6 and subjected to independent sample t-test with SPSS 23.0.No significant difference in Tmax of complanatoside B was observed between the raw and processed groups;however,in the processed group,C_(max) and AUC_(0-12h) of complanatoside B increased obviously(P<0.05),while MRT_(0-12h) decreased from(3.34±0.44)h to(1.81±0.36)h(P<0.05).Cmax[(14.72±11.13)μg·L^(-1)]and MRT0-24[(3.93±0.26)h]of complanatoside A in the raw group were statistically different from those[(35.64±21.99)μg·L^(-1),(1.43±0.24)h]in the processed group(P<0.05).As a result,salt-processing can facilitate the in vivo adsorption and accelerate the excretion of complanatoside A and complanatoside B.