摘要
OBJECTIVE:To investigate the chemical characters of water-extract of Baqi Lingmao formula(巴芪灵猫方,BQLM formula)and its effects on anti-liver injury in model mice and live cells.METHODS:BQLM formula was composed of ten herbal medicines.We determined the contents of alkaloids,saponins,phenolic acids and flavonoid in BQLM formula by UV spectrophotometry.The active components of alkaloids and phenolic acids in BQLM formula were identified by HPLC chromatography.The anti-hepatic injury effects of BQLM formula were investigated with concanavalin A(Con A)-induced hepatitis model of mice,human liver LO2 and Hep G2.2.15 cells.RESULTS:BQLM formula(2 and 10 g/kg,orally)significantly improved the damages of liver tissues and functions caused by Con A in mice,reduced the infiltration of inflammatory cells into liver and inhibited the inflammatory cytokine secretion of interferon-γ,tumor necrosis factor-αand interleukin-6.BQLM formula simultaneously decreased the levels of alanine aminotransferase and aspartate aminotransferase of liver and serum,and recovered the superoxide dismutase and catalase activities of liver to normal levels in Con A-induced hepatic-injury mice.The serum of BQLM formula group stimulated the human liver LO2 cell proliferation in vitro.Further,BQLM formula obviously promoted the proliferation of normal hepatocytes(LO2 cells)and inhibited the hepatocytes death induced by Con A.It also significantly inhibited the proliferation of Hep G2.2.15 cells and decreased the secretion of HBs Ag and HBe Ag in vitro.CONCLUSIONS:BQLM formula has anti-inflammation and anti-hepatitis virus Beffects,and is capable of improving liver injury in vivo and in vitro.
基金
Supported by Science Research Project of Thirteen Five-Year Plan(Optimization of Treatment Scheme of Integrated Traditional Chinese and Western Medicine for Hbeag Negative Chronic Hepatitis B,No.2018ZX10725-504)。
