川芎真空蒸汽润药制备工艺优化及其抗炎镇痛活性研究

Study on the optimization of the preparation technic and pharmacological activity of Chuanxiong Rhizoma

目的应用星点设计-响应面法优化川芎真空蒸汽润药制备工艺,考察润药川芎饮片的抗炎镇痛活性。方法以阿魏酸含量为评价指标,以润药温度、润药时间和真空时间为观察指标,采用响应面法优化川芎饮片润药工艺,并筛出最佳优化方案。采用二甲苯致小鼠耳廓肿胀和冰醋酸致小鼠扭体实验考察润药川芎饮片的抗炎镇痛活性。结果最佳蒸汽润药工艺为:软化温度80℃、软化时间50 min、真空时间45 min。该工艺生产的川芎饮片阿魏酸含量较高,并可减少冰醋酸致小鼠的扭体反应次数、延长潜伏期,抑制二甲苯致小鼠耳廓肿胀反应,明显或部分缓解肿胀程度。结论响应面法优选真空润药工艺简便易行,准确度较好,润药川芎饮片比传统饮片抗炎镇痛效果好,可为川芎饮片的生产提供参考。

Objective To optimize the preparation technic of Chuanxiong Rhizoma with vacuum steam method,and to investigate the anti-inflammatory and analgesic activity of Chuanxiong Rhizoma decoction pieces with Central Composite Design-Response Surface Method.Methods Taking the content of ferulic acid as the evaluation index and the moistening temperature,moistening time and vacuum time as the observation indexes,the moistening technic of Chuanxiong Rhizoma was optimized by Response Surface Method,and selected the optimized plan.The anti-inflammatory and analgesic activities of Chuanxiong Rhizoma were investigated by auricle swelling induced by xylene and writhing induced by glacial acetic acid.Results The optimum vacuum moistening technic was that the softening temperature was 80℃,the softening time was 50 min and the vacuum time was 45 min.The content of ferulic acid in Chuanxiong Rhizoma produced by this technic is highand could decreased the times of wrinkle reaction induced by acetic acid in mice,prolonged the latent period,and obviously or partially inhibitied the ear swelling degree induced byxylene in rats.Conclusions The Response Surface Method technic of Chuanxiong Rhizoma is easy to operate with high accuracy.The vacuum steam treatment was more obvious than traditional technology group.It provides reference for the subsequent production of Chuanxiong Rhizoma decoction pieces and have the certain value for its promotion and application.