利用秀丽隐杆线虫模型评价人工培育肉灵芝浸提物的毒性及抗氧化功效

Evaluation of Toxicity and Antioxidant Efficacy of Artificially Cultivated Meat-Like Ganoderma lucidum Extracts Using Caenorhabditis elegans Model

将人工培育的肉灵芝浸提物作用于秀丽隐杆线虫氧化损伤模型,评价其毒性影响及抗氧化功效.采用紫外全波长扫描法确定浸提物主要成分,采用寿命和产卵量测定法评价浸提物毒性,通过活性氧检测(DCF)法检测自由基含量以评价浸提物抗氧化功效,采用转录组学分析法探究浸提物潜在作用靶点.实验结果表明:肉灵芝浸提物的主要成分为醌类和蛋白质;浸提物无明显毒副作用;2.5 mg/mL浸提物对损伤线虫抗氧化能力显著,可有效调控热激蛋白家族(hsp-16.1,16.11,16.48,16.49)、超氧化物歧化酶(sod-5)、线粒体内膜易位酶(timm-23)、金属硫蛋白-1(mtl-1)和Skp1蛋白家族相关基因的表达.可见人工培育肉灵芝浸提物可通过寿命调节信号通路发挥明显的抗氧化功效,并具有潜在的调控脂肪代谢和促进蛋白合成能力.

The artificially cultivated meat-like Ganoderma lucidum(MGL)extracts were applied to the oxidative damage model of Caenorhabditis elegans to evaluate their toxicity and antioxidant efficacy.The ultraviolet(UV)full wavelength scanning method was used to determine the main components of the extracts,the life span and spawning quantity were used to evaluate the toxicity of the extracts,the free radical content was detected by active oxygen detection(DCF)method to evaluate the antioxidant efficacy of the extracts,the potential acting target of the extracts was explored by the transcriptome analysis.The experimental results show that the main components of the MGL extracts are quinones and proteins,the extracts have no obvious toxic effects,2.5 mg/mL extracts have significant antioxidant capacity against damaged nematodes and can effectively regulate the expression of related genes to the heat shock protein family(hsp-16.1,16.11,16.48,16.49),superoxide dismutase(sod-5),inner mitochondrial membrane(timm-23),metal sulphurin-1(mtl-1),and the Skp1 protein family.The artificially cultivated MGL extracts can exert obvious antioxidant efficacy through the life-regulating signal pathway,and have the potential ability to regulate fat metabolism and promote protein synthesis.