离子色谱电导法测定头孢噻肟钠中钠离子含量

Determination of Sodium Ion in Cefotaxime Sodium by Ion Chromatography

目的:建立离子色谱法测定头孢噻肟钠溶液中钠离子的含量。方法:采用离子色谱法,DionexIonPac^(TM) CS12A(4×250 mm,5μm)阳离子交换色谱柱,以甲基磺酸为淋洗液,流速为1 mL·min^(-1),柱温为30℃,电导检测器检测,测定头孢噻肟钠中钠离子的含量。结果:该方法在0.05~50μg·mL^(-1)浓度范围内线性良好,(r^(2)=0.9998);检测限为0.001μg·mL^(-1),定量限为0.005μg·mL^(-1);平均加样回收率为分别为98.6%,RSD为2.5%(n=9)。结论:该方法准确、简单、灵敏,可用于测定头孢噻肟钠中钠离子含量的测定。

The method for content determination of sodium ion in cefotaxime sodium by ion chromatography was developed.Analysis was performed on a DionexIonPac^(TM) CS12A column(250 mm×4 mm,5μm).The elutriant was methyl sulfonic acid solution.The flow rate was 1.0 mL·min^(-1).The column temperature was set at 30℃.Inhibitory electrical conductivity detector was used.The results showed that a good separation was accepted between sodium ion and other conventional anions.The standard curve was linear in the range of 0.05~50μg·mL^(-1)(r^(2)=0.9998).The average recovery was 98.6%(n=9,RSD=2.5%).This method was accurate,sensitive,specific and was suitable for the determination of sodium ion content in cefotaxime sodium.