脑脊液培养及炎性标志物联合检测对颅内感染诊断价值

The value of combined detection of cerebrospinal fluid culture and inflammatory markers in the diagnosis of intracranial infection

目的:研究近5年安徽省立医院南区神经外科颅内感染患者的脑脊液病原菌分布情况及相关炎性标志物的诊断价值,为神经外科诊治颅内感染提供参考依据。方法:回顾性分析2017年1月-2021年8月某医院神经外科送检脑脊液标本,阳性标本分离菌株使用walkAway40sI全自动微生物鉴仪,并分析同期颅内感染患者及非颅内感染患者血清中的PCT及CRP检测结果。结果:126例阳性菌株中革兰阴性杆菌、革兰阳性球菌和真菌分别占73.02%、25.40%和1.59%。颅内感染患者血清PCT及CRP显著高于非颅内感染患者(P<0.05)。结论:目前脑脊液病原菌培养检出率较低,因此检测技术及手段仍有待提高,但如果结合血清PCT和CRP检测,将有助于提高疾病诊断率。

Objective:To provide a reference treatment of intracranial infection,we studied the distribution of cerebrospinal fluid pathogens and the related inflammatory markers in patients with intracranial infection of Anhui Provincial Hospital in the past 5 years.Methods:Cerebrospinal fluid samples submitted by neurosurgery from January 2017 to August 2021 were analyzed.The isolated strains of positive specimens were identified and tested by walkAway40sI.The patients with intracranial infection or non-intracranial infection were analyzed to test the PCT and CRP expression.Results:In the 126 positive strains,gram-negative bacilli,gram-positive cocci and fungi accounted for 73.02%,25.40%and 1.59%,respectively.The contents of serum PCT and CRP of patients with intracranial infection were significantly higher than those of patients without intracranial infection(P<0.05).Conclusion:The detection rate of pathogenic bacteria in cerebrospinal fluid culture is low,the detection technology and methods still need to be improved,but if combined with serum PCT and CRP detection,the disease diagnosis can be improved.