轴突导向因子G1在肝细胞癌患者血清和组织中的表达及其临床意义

Expression of netrin⁃G1 in serum and tissues of patients with hepatocellular carcinoma and its clinical significance

目的探讨轴突导向因子G1(netrin⁃G1,NTNG1)在肝细胞癌患者血清及组织中的表达水平及其临床意义。方法收集本院病理确诊为肝细胞癌的组织样本63例,采用Western Blot法检测NTNG1蛋白的表达,并分析其表达水平与临床病理特征及预后的关系。同时收集同期本院收治的肝细胞癌、肝炎、健康受试者、其他肿瘤患者血清共90例,采用ELISA法检测血清中NTNG1水平。结果NTNG1在肝细胞癌组织中高表达(P=0.001),是影响肝细胞癌患者总生存期的独立危险因素。肝细胞癌组患者血清中NTNG1水平显著高于其他对照组(均P<0.001),受试者工作特征(receiver operating characteristic,ROC)曲线显示其诊断肝细胞癌的灵敏度和特异度分别为84.62%和76.56%,ROC曲线下面积为0.8251。结论NTNG1在肝细胞癌组织中高表达且与患者预后不良有关,可能是肝细胞癌患者潜在的预后评估标志物。

Objective To investigate the expression of netrin⁃G1(NTNG1)in serum samples and tissues of hepatocellular carcinoma(HCC)patients and its clinical significance.Methods A total of 63 cases of HCC tissue samples confirmed by pathology in Guangxi Medical University Cancer Hospital were collected.The expression of NTNG1 protein was detected by Western blot,and the relationship between the expression level and clinicopathological features as well as prognosis was analyzed.The peripheral serum samples of 90 patients with HCC,hepatitis,healthy subjects,and other tumor patients admitted to the hospital during the same period were collected,and the serum NTNG1 levels was detected by ELISA.Results NTNG1 was highly expressed in HCC tissues(P=0.001),which was an independent risk factor affecting the overall survival of HCC patients.The serum level of NTNG1 in HCC group was significantly higher than that in other control groups(all P<0.001).The receiver operating characteristic(ROC)curve showed that the sensitivity and specificity of HCC were 84.62%and 76.56%,respectively,and the area under the ROC curve was 0.8251.Conclusions NTNG1 is highly expressed in HCC tissues and associated with poor prognosis of HCC patients,which may be a potential prognostic evaluation mark⁃er for HCC patients.