钛纳米管负载米诺环素羟基磷灰石复合涂层的构建

Construction of minocycline hydroxyapatite composite coating on TiO_(2) nanotubes

目的探讨在TiO_(2)纳米管表面构建羟基磷灰石和盐酸米诺环素复合涂层的方法,为钛种植体表面生物化和抗菌化修饰提供新的方法和思路。方法通过阳极氧化法制备钛纳米管,应用模拟体液浸泡法将羟基磷灰石沉积在TiO_(2)纳米管基材表面,构建活性涂层。随后采用滴加法加载盐酸米诺环素,构建复合涂层。场发射扫描电镜观察基材微观结构,X射线光电子能谱仪分析基材元素组成及含量分布,傅立叶红外光谱检测基材基团成分,X射线衍射仪分析基材分子结构。结果场发射扫描电镜见光滑钛片呈镜面状;钛纳米管呈蜂窝状,管径大小均一,大多集中在50~90 nm,其中70 nm管径约占基材表面的2/3;钛纳米管-羟基磷灰石表面有一薄层、云雾状的灰白色沉积物;钛纳米管-羟基磷灰石-米诺环素样品见大块的絮状物将蜂窝状的纳米管和薄层的羟基磷灰石完全覆盖。X射线光电子能谱仪结果显示,在140 eV和350 eV处分别出现磷和钙元素的特征性波峰。X射线衍射仪观察在2θ=34°、38°、40°和53°可见4个显著的骨样磷酸盐衍射峰。傅立叶红外光谱检查在1550 cm^(-1)和3050 cm^(-1)分别出现C=C和苯环的特征性波峰。结论通过模拟体液浸泡法和滴加法,可将羟基磷灰石和米诺环素固定在钛纳米管基材表面,成功构建生物化和抗菌化双重修饰的复合涂层。

Objective To explore the method of constructing hydroxyapatite and minocycline hydrochloride composite coating on the surface of TiO_(2)nanotubesventuallya new method and idea for biological and antibacterial modification of titanium implants.Methods Titanium nanotubes were prepared by anodic oxidation method.Hydroxyapatite was deposited on the surface of TiO_(2)nanotube substrates in a simulated body fluid to construct active coating.Then minocycline hydrochloride was loaded by dropping method to construct composite coating.The microstructure of the substrates was observed by Field emission scanning electron microscope,the elemental composition and content distribution of the substrates were analyzed by X-ray photoelectron spectroscopy,the group composition of the substrates was detected by Fourier transform infrared spectroscopy and the molecular structure of the substrates was analyzed by X-ray diffraction.Results Field emission scanning electron microscope showed that the smooth titanium samples were like mirror shape.Titanium nanotubes had honeycomb structure and the size of diameters was uniform,most of which wconcentrated in 50~90 nm and the diameter of 70 nm accounted for about 2/3 There wa thin layer of grey deposit on the surface of titanium nanotube-hydroxyapatite substrates.The results showed that the titanium nanotube-hydroxyapatite-minocycline samples were covered by large floccules.X-ray photoelectron spectroscopy showed that he distinct peaks of phosphorus and calcium app eared at 140 eV and 350 eV respectively.X-ray diffractometer observed four significant diffraction peaks of bone-like phosphate at 2θ=34°,38°,40°and 53°.Fourier transform infrared spectroscopy showed characteristic peaks of C=C and benzene ring at 1550 cm^(-1)and 3050 cm^(-1)respectively.Conclusion Hydroxyapatite and minocycline can be immobilized on the surface of titanium nanotube substrates by simulated body fluid immersion and dropping method,then the composite coating with biological and antibacterial modification is successfully constructed.