补肾活血方对人胚肺成纤维细胞中IL-17A的影响

Effect of Bushen huoxue recipe on IL-17A in human embryonic lung fibroblasts

目的探讨补肾活血方对人胚肺成纤维细胞的作用机制。方法应用TGF-β1诱导人胚肺成纤维细胞,将实验细胞分为空白组(A组),TGF-β1造模组(B组),TGF-β1处理+最佳浓度含药血清干预1 h组(C组),TGF-β1处理+shRNA-IL-17A组(D组),TGF-β1处理+最佳浓度含药血清干预1 h+IL-17A过表达质粒组(E组),进行相应药物培养后,检测各组细胞的增值率、胶原蛋白及IL-17A表达量的变化。结果B组中人胚肺成纤维细胞的增殖率、胶原蛋白、IL-17A蛋白含量及IL-17A mRNA表达量较A组明显增多(P<0.05);C组、D组细胞的增殖率、胶原蛋白、IL-17A蛋白含量及IL-17AmRNA表达量较B组明显较少;而E组以上各项指标较C组明显增多。结论补肾活血方能有效降解人胚肺成纤维细胞中胶原蛋白的含量,具有较强的降纤作用,主要是通过阻断IL-17A通路降解胶原蛋白而起到抗纤维化作用的。

Objective To investigate the mechanism of Bushen Huoxue decoction(BHXF)on human embryonic lung fibroblasts(HLF).Methods Human embryonic lung fibroblasts were induced by TGF-1.The experimental cells were divided into blank group(Group A),TGF-1 model group(Group B),TGF-1 treatment+best concentration of drug-containing Serum Intervention 1 hour group(Group C),TGF-1 treatment+Shrna-il-17a Group(Group D),tGF-1 treatment plus the best concentration of drug-containing serum interfered 1H+Il-17a overexpression plasmid group(Group E),after the corresponding drug culture,the changes of cell increment rate,Collagen and Il-17a expression were detected.Results The proliferation rate,Collagen,Il-17a and Il-17a Mrna expression of human embryonic lung fibroblasts in group B were significantly higher than those in group a(P<0.05).The cell proliferation rate,Collagen protein,IL-17A protein content and IL-17AmRNA expression in Group C and D were significantly lower than those in Group B,while those in Group E were significantly higher than those in Group C.Conclusion Bushen Huoxue decoction can effectively degrade the content of Collagen in human embryonic lung fibroblasts,and it has a strong defibrination effect,which is mainly through blocking the IL-17A pathway and degrading Collagen.